St Andrews Research Repository

St Andrews University Home
View Item 
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  • Login
JavaScript is disabled for your browser. Some features of this site may not work without it.

Structure of the CRISPR Interference complex CSM reveals key similarities with Cascade

Thumbnail
View/Open
MolecularCell2013.pdf (1.900Mb)
Date
10/10/2013
Author
Rouillon, Christophe
Zhou, Min
Zhang, Jing
Argyris, Politis
Beilsten-Edmands, Victoria
Cannone, Guiseppe
Graham, Shirley
Robinson, Carol
Spagnolo, Laura
White, Malcolm F
Keywords
Clustered Regularly Interspaced Palindromic Repeats (CRISPR) system
Immune system
Prokaryotes
CSM complex
Sulfolobus solfataricus
Cascade complex
Methylation
Acetylation
Phosphorylation
Metadata
Show full item record
Altmetrics Handle Statistics
Altmetrics DOI Statistics
Abstract
The Clustered Regularly Interspaced Palindromic Repeats (CRISPR) system is an adaptive immune system in prokaryotes. Interference complexes encoded by CRISPR-associated (cas) genes utilize small RNAs for homology-directed detection and subsequent degradation of invading genetic elements, and they have been classified into three main types (I–III). Type III complexes share the Cas10 subunit but are subclassifed as type IIIA (CSM) and type IIIB (CMR), depending on their specificity for DNA or RNA targets, respectively. The role of CSM in limiting the spread of conjugative plasmids in Staphylococcus epidermidis was first described in 2008. Here, we report a detailed investigation of the composition and structure of the CSM complex from the archaeon Sulfolobus solfataricus, using a combination of electron microscopy, mass spectrometry, and deep sequencing. This reveals a three-dimensional model for the CSM complex that includes a helical component strikingly reminiscent of the backbone structure of the type I (Cascade) family.
Citation
Rouillon , C , Zhou , M , Zhang , J , Argyris , P , Beilsten-Edmands , V , Cannone , G , Graham , S , Robinson , C , Spagnolo , L & White , M F 2013 , ' Structure of the CRISPR Interference complex CSM reveals key similarities with Cascade ' , Molecular Cell , vol. 52 , no. 1 , pp. 124-134 . https://doi.org/10.1016/j.molcel.2013.08.020
Publication
Molecular Cell
Status
Peer reviewed
DOI
https://doi.org/10.1016/j.molcel.2013.08.020
ISSN
1097-2765
Type
Journal article
Rights
Copyright © 2013 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Collections
  • University of St Andrews Research
URI
http://hdl.handle.net/10023/4556

Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

Advanced Search

Browse

All of RepositoryCommunities & CollectionsBy Issue DateNamesTitlesSubjectsClassificationTypeFunderThis CollectionBy Issue DateNamesTitlesSubjectsClassificationTypeFunder

My Account

Login

Open Access

To find out how you can benefit from open access to research, see our library web pages and Open Access blog. For open access help contact: openaccess@st-andrews.ac.uk.

Accessibility

Read our Accessibility statement.

How to submit research papers

The full text of research papers can be submitted to the repository via Pure, the University's research information system. For help see our guide: How to deposit in Pure.

Electronic thesis deposit

Help with deposit.

Repository help

For repository help contact: Digital-Repository@st-andrews.ac.uk.

Give Feedback

Cookie policy

This site may use cookies. Please see Terms and Conditions.

Usage statistics

COUNTER-compliant statistics on downloads from the repository are available from the IRUS-UK Service. Contact us for information.

© University of St Andrews Library

University of St Andrews is a charity registered in Scotland, No SC013532.

  • Facebook
  • Twitter