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dc.contributor.authorRouillon, Christophe
dc.contributor.authorZhou, Min
dc.contributor.authorZhang, Jing
dc.contributor.authorArgyris, Politis
dc.contributor.authorBeilsten-Edmands, Victoria
dc.contributor.authorCannone, Guiseppe
dc.contributor.authorGraham, Shirley
dc.contributor.authorRobinson, Carol
dc.contributor.authorSpagnolo, Laura
dc.contributor.authorWhite, Malcolm F
dc.date.accessioned2014-04-04T16:01:01Z
dc.date.available2014-04-04T16:01:01Z
dc.date.issued2013-10-10
dc.identifier.citationRouillon , C , Zhou , M , Zhang , J , Argyris , P , Beilsten-Edmands , V , Cannone , G , Graham , S , Robinson , C , Spagnolo , L & White , M F 2013 , ' Structure of the CRISPR Interference complex CSM reveals key similarities with Cascade ' Molecular Cell , vol. 52 , no. 1 , pp. 124-134 . https://doi.org/10.1016/j.molcel.2013.08.020en
dc.identifier.issn1097-2765
dc.identifier.otherPURE: 73771592
dc.identifier.otherPURE UUID: bfafb896-cf33-4d22-acd6-0b28d3604604
dc.identifier.otherScopus: 84885336337
dc.identifier.otherORCID: /0000-0003-1543-9342/work/47136075
dc.identifier.urihttp://hdl.handle.net/10023/4556
dc.description.abstractThe Clustered Regularly Interspaced Palindromic Repeats (CRISPR) system is an adaptive immune system in prokaryotes. Interference complexes encoded by CRISPR-associated (cas) genes utilize small RNAs for homology-directed detection and subsequent degradation of invading genetic elements, and they have been classified into three main types (I–III). Type III complexes share the Cas10 subunit but are subclassifed as type IIIA (CSM) and type IIIB (CMR), depending on their specificity for DNA or RNA targets, respectively. The role of CSM in limiting the spread of conjugative plasmids in Staphylococcus epidermidis was first described in 2008. Here, we report a detailed investigation of the composition and structure of the CSM complex from the archaeon Sulfolobus solfataricus, using a combination of electron microscopy, mass spectrometry, and deep sequencing. This reveals a three-dimensional model for the CSM complex that includes a helical component strikingly reminiscent of the backbone structure of the type I (Cascade) family.
dc.format.extent11
dc.language.isoeng
dc.relation.ispartofMolecular Cellen
dc.rightsCopyright © 2013 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectClustered Regularly Interspaced Palindromic Repeats (CRISPR) systemen
dc.subjectImmune systemen
dc.subjectProkaryotesen
dc.subjectCSM complexen
dc.subjectSulfolobus solfataricusen
dc.subjectCascade complexen
dc.subjectMethylationen
dc.subjectAcetylationen
dc.subjectPhosphorylationen
dc.titleStructure of the CRISPR Interference complex CSM reveals key similarities with Cascadeen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1016/j.molcel.2013.08.020
dc.description.statusPeer revieweden


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