Now showing items 1-8 of 8

    • Acetylation site specificities of lysine deacetylase inhibitors in human cells 

      Schölz, Christian; Weinert, Brian T.; Wagner, Sebastian A.; Beli, Petra; Miyake, Yasuyuki; Qi, Yun; Jensen, Lars J.; Streicher, Werner; McCarthy, Anna Rose; Westwood, Nicholas James; Lain, Sonya; Cox, Jürgen; Matthias, Patrick; Mann, Matthias; Bradner, James E.; Choudhary, Chunaram (2015) - Journal article
      Lysine deacetylases inhibitors (KDACIs) are used in basic research, and many are being investigated in clinical trials for treatment of cancer and other diseases. However, their specificities in cells are incompletely ...
    • Characterisation of Sulfolobus solfataricus Ard1, a promiscuous N-acetyltransferase 

      Mackay, Dale Tara (University of St Andrews, 2008-06-27) - Thesis
      Compaction of DNA into chromatin is an important feature of every living cell. This compaction phenomenon is brought about and maintained by a variety of DNA binding proteins, which have evolved to suit the specific needs ...
    • Dimer-dimer stacking interactions are important for nucleic acid binding by the archaeal chromatin protein Alba 

      Jelinska, Clare; Petrovic-Stojanovska, Biljana; Ingledew, W John; White, Malcolm F (2010-04-01) - Journal article
      Archaea use a variety of small basic proteins to package their DNA. One of the most widespread and highly conserved is the Alba (Sso10b) protein. Alba interacts with both DNA and RNA in vitro, and we show in the present ...
    • Discovery, in vivo activity, and mechanism of action of a small-molecule p53 activator 

      Lain, Sonia; Hollick, Jonathan J.; Campbell, Johanna; Staples, Oliver D.; Higgins, Maureen; Aoubala, Mustapha; McCarthy, Anna; Appleyard, Virginia; Murray, Karen E.; Baker, Lee; Thompson, Alastair; Mathers, Joanne; Holland, Stephen J.; Stark, Michael J. R.; Pass, Georgia; Woods, Julie; Lane, David P.; Westwood, Nicholas J. (2008-05-06) - Journal article
      We have carried out a cell-based screen aimed at discovering small molecules that activate p53 and have the potential to decrease tumor growth. Here, we describe one of our hit compounds, tenovin-1, along with a more ...
    • Extensive lysine methylation in hyperthermophilic crenarchaea : potential implications for protein stability and recombinant enzymes 

      Botting, Catherine H.; Talbot, Paul; Paytubi, Sonia; White, Malcolm F. (2010) - Journal article
      In eukarya and bacteria, lysine methylation is relatively rare and is catalysed by sequence-specific lysine methyltransferases that typically have only a single-protein target. Using RNA polymerase purified from the ...
    • The histone chaperones Vps75 and Nap1 form ring-like, tetrameric structures in solution 

      Bowman, Andrew; Hammond, Colin M.; Stirling, Andrew; Ward, Richard; Shang, Weifeng; El Mkami, Hassane; Robinson, David A.; Svergun, Dmitri I.; Norman, David G.; Owen-Hughes, Tom (2014) - Journal article
      NAP-1 fold histone chaperones play an important role in escorting histones to and from sites of nucleosome assembly and disassembly. The two NAP-1 fold histone chaperones in budding yeast, Vps75 and Nap1, have previously ...
    • A proteomic approach to analyze the aspirin-mediated lysine acetylome 

      Tatham, Michael H.; Cole, Christian; Scullian, Paul; Wilkie, Ross; Westwood, Nicholas J.; Stark, Lesley A.; Hay, Ronald T. (2017-02-01) - Journal article
      Aspirin, or acetylsalicylic acid is widely used to control pain, inflammation and fever. Important to this function is its ability to irreversibly acetylate cyclooxygenases at active site serines. Aspirin has the potential ...
    • Structure of the CRISPR Interference complex CSM reveals key similarities with Cascade 

      Rouillon, Christophe; Zhou, Min; Zhang, Jing; Argyris, Politis; Beilsten-Edmands, Victoria; Cannone, Guiseppe; Graham, Shirley; Robinson, Carol; Spagnolo, Laura; White, Malcolm F (2013-10-10) - Journal article
      The Clustered Regularly Interspaced Palindromic Repeats (CRISPR) system is an adaptive immune system in prokaryotes. Interference complexes encoded by CRISPR-associated (cas) genes utilize small RNAs for homology-directed ...