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Axonal localization of integrins in the CNS is neuronal type and age dependent

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Date
07/2016
Author
Andrews, Melissa Renee
Soleman, Sara
Cheah, Menghon
Tumbarello, David
Mason, Matthew
Moloney, Elizabeth
Verhaagen, Joost
Bensadoun, Jean-Charles
Schneider, Bernard
Aebischer, Patrick
Fawcett, James
Keywords
Adeno-associated virus
Axon
Axon initial segment
Dorsal root ganglia
Integrin
Lentivirus
Retinal ganglion-cells
Sensorimotor cortex
QH426 Genetics
RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Neuroscience(all)
NDAS
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Abstract
The regenerative ability of CNS axons decreases with age however this ability remains largely intact in PNS axons throughout adulthood. These differences are likely to correspond with age-related silencing of proteins necessary for axon growth and elongation. In previous studies, it has been shown that reintroduction of the alpha9 integrin subunit (tenascin-C receptor, α9) that is downregulated in adult CNS can improve neurite outgrowth and sensory axon regeneration after a dorsal rhizotomy or a dorsal column crush spinal cord lesion. In the current study, we demonstrate that virally-expressed integrins (α9, α6, or β1 integrin) in the adult rat sensorimotor cortex and adult red nucleus are excluded from axons following neuronal transduction. Attempts to stimulate transport by inclusion of a cervical spinal injury and thus an upregulation of extracellular matrix molecules at the lesion site, or co-transduction with its binding partner, β1 integrin, did not induce integrin localization within axons. In contrast, virally-expressed α9 integrin in developing rat cortex (postnatal day 5 or 10) demonstrated clear localization of integrins in cortical axons revealed by the presence of integrin in the axons of the corpus callosum and internal capsule as well as in the neuronal cell body. Furthermore, examination of dorsal root ganglia neurons and retinal ganglion cells demonstrated integrin localization both within peripheral nerve as well as dorsal root axons and within optic nerve axons, respectively. Together, our results suggest a differential ability for in vivo axonal transport of transmembrane proteins dependent on neuronal age and subtype.
Citation
Andrews , M R , Soleman , S , Cheah , M , Tumbarello , D , Mason , M , Moloney , E , Verhaagen , J , Bensadoun , J-C , Schneider , B , Aebischer , P & Fawcett , J 2016 , ' Axonal localization of integrins in the CNS is neuronal type and age dependent ' , eNeuro , vol. 3 , no. 4 , e0029-16.2016 . https://doi.org/10.1523/ENEURO.0029-16.2016
Publication
eNeuro
Status
Peer reviewed
DOI
https://doi.org/10.1523/ENEURO.0029-16.2016
ISSN
2373-2822
Type
Journal article
Rights
Copyright © 2016 Andrews et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Description
This work was supported by the International Foundation for Research in Paraplegia (MRA), the Bryon Riesch Paralysis Foundation (MRA), the American Association of Anatomists (MRA), the Christopher and Dana Reeve Foundation (JWF), the Medical Research Council (JWF), the Plasticise European Network (seventh framework program) (JWF) and the NIHR Cambridge Biomedical Research Centre.
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  • University of St Andrews Research
URI
http://hdl.handle.net/10023/9250

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