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dc.contributor.authorAndrews, Melissa Renee
dc.contributor.authorSoleman, Sara
dc.contributor.authorCheah, Menghon
dc.contributor.authorTumbarello, David
dc.contributor.authorMason, Matthew
dc.contributor.authorMoloney, Elizabeth
dc.contributor.authorVerhaagen, Joost
dc.contributor.authorBensadoun, Jean-Charles
dc.contributor.authorSchneider, Bernard
dc.contributor.authorAebischer, Patrick
dc.contributor.authorFawcett, James
dc.date.accessioned2016-08-03T16:30:21Z
dc.date.available2016-08-03T16:30:21Z
dc.date.issued2016-07
dc.identifier.citationAndrews , M R , Soleman , S , Cheah , M , Tumbarello , D , Mason , M , Moloney , E , Verhaagen , J , Bensadoun , J-C , Schneider , B , Aebischer , P & Fawcett , J 2016 , ' Axonal localization of integrins in the CNS is neuronal type and age dependent ' , eNeuro , vol. 3 , no. 4 , e0029-16.2016 . https://doi.org/10.1523/ENEURO.0029-16.2016en
dc.identifier.issn2373-2822
dc.identifier.otherPURE: 244180179
dc.identifier.otherPURE UUID: 06a8ffcc-7ac1-472a-812e-6d8780c47f9a
dc.identifier.otherScopus: 85019538563
dc.identifier.otherWOS: 000391928700003
dc.identifier.urihttps://hdl.handle.net/10023/9250
dc.descriptionThis work was supported by the International Foundation for Research in Paraplegia (MRA), the Bryon Riesch Paralysis Foundation (MRA), the American Association of Anatomists (MRA), the Christopher and Dana Reeve Foundation (JWF), the Medical Research Council (JWF), the Plasticise European Network (seventh framework program) (JWF) and the NIHR Cambridge Biomedical Research Centre.en
dc.description.abstractThe regenerative ability of CNS axons decreases with age however this ability remains largely intact in PNS axons throughout adulthood. These differences are likely to correspond with age-related silencing of proteins necessary for axon growth and elongation. In previous studies, it has been shown that reintroduction of the alpha9 integrin subunit (tenascin-C receptor, α9) that is downregulated in adult CNS can improve neurite outgrowth and sensory axon regeneration after a dorsal rhizotomy or a dorsal column crush spinal cord lesion. In the current study, we demonstrate that virally-expressed integrins (α9, α6, or β1 integrin) in the adult rat sensorimotor cortex and adult red nucleus are excluded from axons following neuronal transduction. Attempts to stimulate transport by inclusion of a cervical spinal injury and thus an upregulation of extracellular matrix molecules at the lesion site, or co-transduction with its binding partner, β1 integrin, did not induce integrin localization within axons. In contrast, virally-expressed α9 integrin in developing rat cortex (postnatal day 5 or 10) demonstrated clear localization of integrins in cortical axons revealed by the presence of integrin in the axons of the corpus callosum and internal capsule as well as in the neuronal cell body. Furthermore, examination of dorsal root ganglia neurons and retinal ganglion cells demonstrated integrin localization both within peripheral nerve as well as dorsal root axons and within optic nerve axons, respectively. Together, our results suggest a differential ability for in vivo axonal transport of transmembrane proteins dependent on neuronal age and subtype.
dc.format.extent14
dc.language.isoeng
dc.relation.ispartofeNeuroen
dc.rightsCopyright © 2016 Andrews et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.en
dc.subjectAdeno-associated virusen
dc.subjectAxonen
dc.subjectAxon initial segmenten
dc.subjectDorsal root gangliaen
dc.subjectIntegrinen
dc.subjectLentivirusen
dc.subjectRetinal ganglion-cellsen
dc.subjectSensorimotor cortexen
dc.subjectQH426 Geneticsen
dc.subjectRC0321 Neuroscience. Biological psychiatry. Neuropsychiatryen
dc.subjectNeuroscience(all)en
dc.subjectNDASen
dc.subject.lccQH426en
dc.subject.lccRC0321en
dc.titleAxonal localization of integrins in the CNS is neuronal type and age dependenten
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.identifier.doihttps://doi.org/10.1523/ENEURO.0029-16.2016
dc.description.statusPeer revieweden


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