St Andrews Research Repository

St Andrews University Home
View Item 
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  • Login
JavaScript is disabled for your browser. Some features of this site may not work without it.

Antitumour activity of the novel flavonoid oncamex in preclinical breast cancer models

Thumbnail
View/Open
Mart_nez_P_rez_2016_AntitumourActivity_BJC_CC.pdf (2.389Mb)
Date
13/04/2016
Author
Martínez-Pérez, Carlos
Ward, Carol
Turnbull, Arran K
Mullen, Peter
Cook, Graham
Meehan, James
Jarman, Edward J
Thomson, Patrick I T
Campbell, Colin J
McPhail, Donald
Harrison, David James
Langdon, Simon P
Keywords
Novel flavonoids
Preclinical models of cancer
ROS modulation
Breast cancer
Animal models of cancer
Natural products
SAR studies
Xenograft models
Novel antitumour agents
RB Pathology
RC0254 Neoplasms. Tumors. Oncology (including Cancer)
NDAS
Metadata
Show full item record
Altmetrics Handle Statistics
Altmetrics DOI Statistics
Abstract
Background: The natural polyphenol myricetin induces cell cycle arrest and apoptosis in preclinical cancer models. We hypothesised that myricetin-derived flavonoids with enhanced redox properties, improved cell uptake and mitochondrial targeting might have increased potential as antitumour agents. Methods: We studied the effect of a second-generation flavonoid analogue, Oncamex, in a panel of 7 breast cancer cell lines, applying western blotting, gene expression analysis, fluorescence microscopy, and immunohistochemistry to xenograft tissue to investigate its mechanism of action. Results: Proliferation assays showed that Oncamex: treatment for 8 h reduced cell viability and induced cytotoxicity and apoptosis, concomitant with increased caspase activation. Microarray analysis showed that Oncamex was associated with changes in expression of genes controlling cell cycle and apoptosis . Fluorescence microscopy showed the compound’s mitochondrial targeting and ROS-modulating properties, inducing superoxide production at concentrations associated with anti-proliferative effects. A preliminary in vivo study in mice implanted with the MDA-MB-231 breast cancer xenograft showed that Oncamex inhibited tumour growth, reduced tissue viability and Ki-67 proliferation, with no overall systemic toxicity. Conclusion: Oncamex is a novel flavonoid capable of specific mitochondrial delivery and redox modulation. It has shown antitumour activity in preclinical models of breast cancer, supporting the potential of this prototypic candidate for its continued development as an anticancer agent.
Citation
Martínez-Pérez , C , Ward , C , Turnbull , A K , Mullen , P , Cook , G , Meehan , J , Jarman , E J , Thomson , P I T , Campbell , C J , McPhail , D , Harrison , D J & Langdon , S P 2016 , ' Antitumour activity of the novel flavonoid oncamex in preclinical breast cancer models ' , British Journal of Cancer , vol. 114 , no. 8 , pp. 905-916 . https://doi.org/10.1038/bjc.2016.6
Publication
British Journal of Cancer
Status
Peer reviewed
DOI
https://doi.org/10.1038/bjc.2016.6
ISSN
0007-0920
Type
Journal article
Rights
This work is licensed under the Creative Commons Attribution-Non-Commercial-Share Alike 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
Description
We thank SULSA (Scottish Universities Life Science Alliance) for supporting this project through a SULSA BioSkape Industry PhD Studentship and Antoxis Limited for providing additional funding. We also thank the 7th Framework Programme of the European Union (METOXIA project; HEALTH-F2-2009-222741) for support.
Collections
  • University of St Andrews Research
URI
http://hdl.handle.net/10023/8579

Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

Related items

Showing items related by title, author, creator and subject.

  • "Will it affect our chances of having children?" and feeling "like a ticking bomb" —the fertility concerns and fears of cancer progression and recurrence in cancer treatment decision-making among young women diagnosed with gynaecological or breast cancer 

    Sobota, Aleksandra; Ozakinci, Gozde (2021-06-02) - Journal article
    Objective: Cancer treatment decision making process is particularly fraught with challenges for young women because the treatment can affect their reproductive potential. Among many factors affecting the process, fears of ...
  • Determinants of fertility issues experienced by young women diagnosed with breast or gynaecological cancer - a quantitative, cross-cultural study 

    Sobota, Aleksandra; Ozakinci, Gozde (2018-09-06) - Journal article
    Background. Although there is a recognition of the importance of fertility to young women with cancer, we do not know who is at risk of distress related to fertility issues following diagnosis. We investigated the determinants ...
  • A systematic review of the supportive care needs of people living with and beyond cancer of the colon and/or rectum 

    Kotronoulas, Grigorios; Papadopoulou, Constantina; Cunningham, Kathryn; Simpson, Mhairi; Maguire, Roma (2017-08) - Journal item
    Purpose: Gaining a clear understanding of the health needs and concerns of people with cancer of the colon and/or rectum can help identify ways to offer a comprehensive care package. Our aim was to systematically assess ...
Advanced Search

Browse

All of RepositoryCommunities & CollectionsBy Issue DateNamesTitlesSubjectsClassificationTypeFunderThis CollectionBy Issue DateNamesTitlesSubjectsClassificationTypeFunder

My Account

Login

Open Access

To find out how you can benefit from open access to research, see our library web pages and Open Access blog. For open access help contact: openaccess@st-andrews.ac.uk.

Accessibility

Read our Accessibility statement.

How to submit research papers

The full text of research papers can be submitted to the repository via Pure, the University's research information system. For help see our guide: How to deposit in Pure.

Electronic thesis deposit

Help with deposit.

Repository help

For repository help contact: Digital-Repository@st-andrews.ac.uk.

Give Feedback

Cookie policy

This site may use cookies. Please see Terms and Conditions.

Usage statistics

COUNTER-compliant statistics on downloads from the repository are available from the IRUS-UK Service. Contact us for information.

© University of St Andrews Library

University of St Andrews is a charity registered in Scotland, No SC013532.

  • Facebook
  • Twitter