The Scottish Structural Proteomics Facility : targets, methods and outputs

The Scottish Structural Proteomics Facility was funded to develop a laboratory scale approach to high throughput structure determination. The effort was successful in that over 40 structures were determined. These structures and the methods harnessed to obtain them are reported here. This report reflects on the value of automation but also on the continued requirement for a high degree of scientific and technical expertise. The efficiency of the process poses challenges to the current paradigm of structural analysis and publication. In the 5 year period we published ten peer-reviewed papers reporting structural data arising from the pipeline. Nevertheless, the number of structures solved exceeded our ability to analyse and publish each new finding. By reporting the experimental details and depositing the structures we hope to maximize the impact of the project by allowing others to follow up the relevant biology.

Citation

Oke , M , Carter , LG , Johnson , KA , Liu , H , McMahon , SA , Yan , X , Kerou , M , Weikart , ND , Kadi , N , Sheikh , MA , Schmelz , S , Dorward , M , Zawadzki , M , Cozens , C , Falconer , H , Powers , H , Overton , IM , van Niekerk , CA , Peng , X , Patel , P , Garrett , RA , Prangishvili , D , Botting , CH , Coote , PJ , Dryden , DT , Barton , GJ , Schwarz-Linek , U , Challis , GL , Taylor , GL , White , MF & Naismith , JH 2010 , ' The Scottish Structural Proteomics Facility : targets, methods and outputs ' , Journal of Structural and Functional Genomics , vol. 11 , no. 2 , pp. 167-180 . https://doi.org/10.1007/s10969-010-9090-y

Publication

Journal of Structural and Functional Genomics

Status

Peer reviewed

DOI

https://doi.org/10.1007/s10969-010-9090-y

ISSN

1345-711X

Type

Journal article

Rights

© The Author(s) 2010. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

Description

The SSPF was supported by grants from The Scottish Funding Council (references SSPF and SULSA), The Biotechnology and Biological Sciences Research Council (reference BB/S/B14450), European Union under framework 7 (reference Aeropath).