Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis
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In the field of biomedical optics, Raman spectroscopy is a powerful tool for probing the chemical composition of biological samples. In particular, fiber Raman probes play a crucial role for in vivo and ex vivo tissue analysis. However, the high-fluorescence background typically contributed by the auto fluorescence from both a tissue sample and the fiber-probe interferes strongly with the relatively weak Raman signal. Here we demonstrate the implementation of wavelength-modulated Raman spectroscopy (WMRS) to suppress the fluorescence background while analyzing tissues using fiber Raman probes. We have observed a significant signal-to-noise ratio enhancement in the Raman bands of bone tissue, which have a relatively high fluorescence background. Implementation of WMRS in fiber-probe-based bone tissue study yielded usable Raman spectra in a relatively short acquisition time (∼30 s), notably without any special sample preparation stage. Finally, we have validated its capability to suppress fluorescence on other tissue samples such as adipose tissue derived from four different species.
Balagopal , B , Ashok , P C , Mazilu , M , Riches , A C , Herrington , C S & Dholakia , K 2012 , ' Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis ' Journal of Biomedical Optics , vol 17 , no. 7 , 077006 . DOI: 10.1117/1.JBO.17.7.077006
Journal of Biomedical Optics
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The work was funded by CR-UK/EPSRC/MRC/DoH (England) imaging programme.
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