Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high fat diet
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The aim of this study was to investigate whether the presence of endogenous estradiol alters the effects of a high-fat (HF) diet on activity/expression of the cardiac Na+/K+-ATPase, via PI3K/IRS and RhoA/ROCK signalling cascades in female rats. For this study, female Wistar rats (8 weeks old, 150–200 g) were fed a standard diet or a HF diet (balanced diet for laboratory rats enriched with 42% fat) for 10 weeks. The results show that rats fed a HF diet exhibited a decrease in phosphorylation of the α1 subunit of Na+/K+-ATPase by 30% (p < 0.05), expression of total α1 subunit of Na+/K+-ATPase by 31% (p < 0.05), and association of IRS1 with p85 subunit of PI3K by 42% (p < 0.05), while the levels of cardiac RhoA and ROCK2 were significantly increased by 84% (p < 0.01) and 62% (p < 0.05), respectively. Our results suggest that a HF diet alters cardiac Na+/K+-ATPase expression via molecular mechanisms involving RhoA/ROCK and IRS-1/PI3K signalling in female rats.
Jovanovic , A , Obradovic , M , Sudar-Milovanovic , E , Stewart , A J , Pitt , S J , Alvantic , D , Aleksic , E & Isenovic , E R 2017 , ' Changes in cardiac Na + /K + -ATPase expression and activity in female rats fed a high fat diet ' Molecular and Cellular Biochemistry , vol. 436 , no. 1-2 , pp. 49-58 . https://doi.org/10.1007/s11010-017-3077-y
Molecular and Cellular Biochemistry
© 2017, Springer. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at link.springer.com / https://doi.org/10.1007/s11010-017-3077-y
DescriptionThis work is supported by the Grant Nos. 173033 and III41028 from the Ministry of Science, Republic of Serbia.
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