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Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high fat diet
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dc.contributor.author | Jovanovic, Aleksandra | |
dc.contributor.author | Obradovic, Milan | |
dc.contributor.author | Sudar-Milovanovic, Emina | |
dc.contributor.author | Stewart, Alan J. | |
dc.contributor.author | Pitt, Samantha J. | |
dc.contributor.author | Alvantic, Dragan | |
dc.contributor.author | Aleksic, Ema | |
dc.contributor.author | Isenovic, Esma R. | |
dc.date.accessioned | 2018-05-31T23:33:03Z | |
dc.date.available | 2018-05-31T23:33:03Z | |
dc.date.issued | 2017-12 | |
dc.identifier | 250088888 | |
dc.identifier | fa5e0323-9ffe-4f5b-8906-b2fe51ca20f6 | |
dc.identifier | 85020098496 | |
dc.identifier | 000414696600006 | |
dc.identifier.citation | Jovanovic , A , Obradovic , M , Sudar-Milovanovic , E , Stewart , A J , Pitt , S J , Alvantic , D , Aleksic , E & Isenovic , E R 2017 , ' Changes in cardiac Na + /K + -ATPase expression and activity in female rats fed a high fat diet ' , Molecular and Cellular Biochemistry , vol. 436 , no. 1-2 , pp. 49-58 . https://doi.org/10.1007/s11010-017-3077-y | en |
dc.identifier.issn | 0300-8177 | |
dc.identifier.other | ORCID: /0000-0003-4580-1840/work/60195806 | |
dc.identifier.other | ORCID: /0000-0003-2257-1595/work/60196233 | |
dc.identifier.uri | https://hdl.handle.net/10023/13651 | |
dc.description | This work is supported by the Grant Nos. 173033 and III41028 from the Ministry of Science, Republic of Serbia. | en |
dc.description.abstract | The aim of this study was to investigate whether the presence of endogenous estradiol alters the effects of a high-fat (HF) diet on activity/expression of the cardiac Na+/K+-ATPase, via PI3K/IRS and RhoA/ROCK signalling cascades in female rats. For this study, female Wistar rats (8 weeks old, 150–200 g) were fed a standard diet or a HF diet (balanced diet for laboratory rats enriched with 42% fat) for 10 weeks. The results show that rats fed a HF diet exhibited a decrease in phosphorylation of the α1 subunit of Na+/K+-ATPase by 30% (p < 0.05), expression of total α1 subunit of Na+/K+-ATPase by 31% (p < 0.05), and association of IRS1 with p85 subunit of PI3K by 42% (p < 0.05), while the levels of cardiac RhoA and ROCK2 were significantly increased by 84% (p < 0.01) and 62% (p < 0.05), respectively. Our results suggest that a HF diet alters cardiac Na+/K+-ATPase expression via molecular mechanisms involving RhoA/ROCK and IRS-1/PI3K signalling in female rats. | |
dc.format.extent | 10 | |
dc.format.extent | 256921 | |
dc.language.iso | eng | |
dc.relation.ispartof | Molecular and Cellular Biochemistry | en |
dc.subject | High-fat diet | en |
dc.subject | Estradiol | en |
dc.subject | NA+/K+-ATPase | en |
dc.subject | Obesity | en |
dc.subject | RhoA/ROCK signalling | en |
dc.subject | Female | en |
dc.subject | QH301 Biology | en |
dc.subject | QH426 Genetics | en |
dc.subject | NDAS | en |
dc.subject | SDG 3 - Good Health and Well-being | en |
dc.subject.lcc | QH301 | en |
dc.subject.lcc | QH426 | en |
dc.title | Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high fat diet | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. Institute of Behavioural and Neural Sciences | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. Cellular Medicine Division | en |
dc.identifier.doi | 10.1007/s11010-017-3077-y | |
dc.description.status | Peer reviewed | en |
dc.date.embargoedUntil | 2018-05-31 |
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