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dc.contributor.authorJovanovic, Aleksandra
dc.contributor.authorObradovic, Milan
dc.contributor.authorSudar-Milovanovic, Emina
dc.contributor.authorStewart, Alan J.
dc.contributor.authorPitt, Samantha J.
dc.contributor.authorAlvantic, Dragan
dc.contributor.authorAleksic, Ema
dc.contributor.authorIsenovic, Esma R.
dc.date.accessioned2018-05-31T23:33:03Z
dc.date.available2018-05-31T23:33:03Z
dc.date.issued2017-12
dc.identifier.citationJovanovic , A , Obradovic , M , Sudar-Milovanovic , E , Stewart , A J , Pitt , S J , Alvantic , D , Aleksic , E & Isenovic , E R 2017 , ' Changes in cardiac Na + /K + -ATPase expression and activity in female rats fed a high fat diet ' , Molecular and Cellular Biochemistry , vol. 436 , no. 1-2 , pp. 49-58 . https://doi.org/10.1007/s11010-017-3077-yen
dc.identifier.issn0300-8177
dc.identifier.otherPURE: 250088888
dc.identifier.otherPURE UUID: fa5e0323-9ffe-4f5b-8906-b2fe51ca20f6
dc.identifier.otherScopus: 85020098496
dc.identifier.otherORCID: /0000-0003-4580-1840/work/60195806
dc.identifier.otherORCID: /0000-0003-2257-1595/work/60196233
dc.identifier.otherWOS: 000414696600006
dc.identifier.urihttp://hdl.handle.net/10023/13651
dc.descriptionThis work is supported by the Grant Nos. 173033 and III41028 from the Ministry of Science, Republic of Serbia.en
dc.description.abstractThe aim of this study was to investigate whether the presence of endogenous estradiol alters the effects of a high-fat (HF) diet on activity/expression of the cardiac Na+/K+-ATPase, via PI3K/IRS and RhoA/ROCK signalling cascades in female rats. For this study, female Wistar rats (8 weeks old, 150–200 g) were fed a standard diet or a HF diet (balanced diet for laboratory rats enriched with 42% fat) for 10 weeks. The results show that rats fed a HF diet exhibited a decrease in phosphorylation of the α1 subunit of Na+/K+-ATPase by 30% (p < 0.05), expression of total α1 subunit of Na+/K+-ATPase by 31% (p < 0.05), and association of IRS1 with p85 subunit of PI3K by 42% (p < 0.05), while the levels of cardiac RhoA and ROCK2 were significantly increased by 84% (p < 0.01) and 62% (p < 0.05), respectively. Our results suggest that a HF diet alters cardiac Na+/K+-ATPase expression via molecular mechanisms involving RhoA/ROCK and IRS-1/PI3K signalling in female rats.
dc.format.extent10
dc.language.isoeng
dc.relation.ispartofMolecular and Cellular Biochemistryen
dc.rights© 2017, Springer. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at link.springer.com / https://doi.org/10.1007/s11010-017-3077-yen
dc.subjectHigh-fat dieten
dc.subjectEstradiolen
dc.subjectNA+/K+-ATPaseen
dc.subjectObesityen
dc.subjectRhoA/ROCK signallingen
dc.subjectFemaleen
dc.subjectQH301 Biologyen
dc.subjectQH426 Geneticsen
dc.subjectNDASen
dc.subject.lccQH301en
dc.subject.lccQH426en
dc.titleChanges in cardiac Na+/K+-ATPase expression and activity in female rats fed a high fat dieten
dc.typeJournal articleen
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.Cellular Medicine Divisionen
dc.identifier.doihttps://doi.org/10.1007/s11010-017-3077-y
dc.description.statusPeer revieweden
dc.date.embargoedUntil2018-05-31


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