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2′-alkynylnucleotides : a sequence- and spin label-flexible strategy for EPR Spectroscopy in DNA

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Haughland_2016_EPR_JACS_9069.pdf (1.355Mb)
Date
27/07/2016
Author
Haugland, Marius M.
El-Sagheer, Afaf H.
Porter, Rachel J.
Peña, Javier
Brown, Tom
Anderson, Edward A.
Lovett, Janet E.
Keywords
QD Chemistry
Biochemistry
Catalysis
Colloid and Surface Chemistry
Chemistry(all)
NDAS
BDC
R2C
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Abstract
Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2′-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron-electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information.
Citation
Haugland , M M , El-Sagheer , A H , Porter , R J , Peña , J , Brown , T , Anderson , E A & Lovett , J E 2016 , ' 2′-alkynylnucleotides : a sequence- and spin label-flexible strategy for EPR Spectroscopy in DNA ' , Journal of the American Chemical Society , vol. 138 , no. 29 , pp. 9069-9072 . https://doi.org/10.1021/jacs.6b05421
Publication
Journal of the American Chemical Society
Status
Peer reviewed
DOI
https://doi.org/10.1021/jacs.6b05421
ISSN
0002-7863
Type
Journal article
Rights
Copyright © 2016 American Chemical Society. ACS AuthorChoice - This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Description
TB and AHE-S thank the BBSRC (sLOLA grant BB/J001694/1). EAA thanks the EPSRC (EP/K005391/1, EP/M019195/1). JEL thanks the EPSRC (EP/LO22044/1), and the Royal Society for a University Research Fellowship and grant RF120645. We thank the Wellcome Trust for the Q-band EPR spectrometer (099149/Z/12/Z), the EPSRC National EPR Service for spectrometer time, and Dr Hassane El Mkami for technical assistance.
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  • University of St Andrews Research
URI
http://hdl.handle.net/10023/9364

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