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dc.contributor.authorHaugland, Marius M.
dc.contributor.authorEl-Sagheer, Afaf H.
dc.contributor.authorPorter, Rachel J.
dc.contributor.authorPeña, Javier
dc.contributor.authorBrown, Tom
dc.contributor.authorAnderson, Edward A.
dc.contributor.authorLovett, Janet E.
dc.identifier.citationHaugland , M M , El-Sagheer , A H , Porter , R J , Peña , J , Brown , T , Anderson , E A & Lovett , J E 2016 , ' 2′-alkynylnucleotides : a sequence- and spin label-flexible strategy for EPR Spectroscopy in DNA ' , Journal of the American Chemical Society , vol. 138 , no. 29 , pp. 9069-9072 .
dc.identifier.otherPURE: 245029241
dc.identifier.otherPURE UUID: 1378c09e-52ea-4146-8ece-5d4be0c4a2cb
dc.identifier.otherScopus: 84979955517
dc.identifier.otherORCID: /0000-0002-3561-450X/work/40301303
dc.identifier.otherWOS: 000380730000020
dc.descriptionTB and AHE-S thank the BBSRC (sLOLA grant BB/J001694/1). EAA thanks the EPSRC (EP/K005391/1, EP/M019195/1). JEL thanks the EPSRC (EP/LO22044/1), and the Royal Society for a University Research Fellowship and grant RF120645. We thank the Wellcome Trust for the Q-band EPR spectrometer (099149/Z/12/Z), the EPSRC National EPR Service for spectrometer time, and Dr Hassane El Mkami for technical assistance.en
dc.description.abstractElectron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2′-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron-electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information.
dc.relation.ispartofJournal of the American Chemical Societyen
dc.rightsCopyright © 2016 American Chemical Society. ACS AuthorChoice - This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.en
dc.subjectQD Chemistryen
dc.subjectColloid and Surface Chemistryen
dc.title2′-alkynylnucleotides : a sequence- and spin label-flexible strategy for EPR Spectroscopy in DNAen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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