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2′-alkynylnucleotides : a sequence- and spin label-flexible strategy for EPR Spectroscopy in DNA

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dc.contributor.authorHaugland, Marius M.
dc.contributor.authorEl-Sagheer, Afaf H.
dc.contributor.authorPorter, Rachel J.
dc.contributor.authorPeña, Javier
dc.contributor.authorBrown, Tom
dc.contributor.authorAnderson, Edward A.
dc.contributor.authorLovett, Janet E.
dc.date.accessioned2016-08-23T14:30:24Z
dc.date.available2016-08-23T14:30:24Z
dc.date.issued2016-07-27
dc.identifier.citationHaugland , M M , El-Sagheer , A H , Porter , R J , Peña , J , Brown , T , Anderson , E A & Lovett , J E 2016 , ' 2′-alkynylnucleotides : a sequence- and spin label-flexible strategy for EPR Spectroscopy in DNA ' , Journal of the American Chemical Society , vol. 138 , no. 29 , pp. 9069-9072 . https://doi.org/10.1021/jacs.6b05421en
dc.identifier.issn0002-7863
dc.identifier.otherPURE: 245029241
dc.identifier.otherPURE UUID: 1378c09e-52ea-4146-8ece-5d4be0c4a2cb
dc.identifier.otherScopus: 84979955517
dc.identifier.otherORCID: /0000-0002-3561-450X/work/40301303
dc.identifier.otherWOS: 000380730000020
dc.identifier.urihttps://hdl.handle.net/10023/9364
dc.descriptionTB and AHE-S thank the BBSRC (sLOLA grant BB/J001694/1). EAA thanks the EPSRC (EP/K005391/1, EP/M019195/1). JEL thanks the EPSRC (EP/LO22044/1), and the Royal Society for a University Research Fellowship and grant RF120645. We thank the Wellcome Trust for the Q-band EPR spectrometer (099149/Z/12/Z), the EPSRC National EPR Service for spectrometer time, and Dr Hassane El Mkami for technical assistance.en
dc.description.abstractElectron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2′-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron-electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information.
dc.format.extent4
dc.language.isoeng
dc.relation.ispartofJournal of the American Chemical Societyen
dc.rightsCopyright © 2016 American Chemical Society. ACS AuthorChoice - This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.en
dc.subjectQD Chemistryen
dc.subjectBiochemistryen
dc.subjectCatalysisen
dc.subjectColloid and Surface Chemistryen
dc.subjectChemistry(all)en
dc.subjectNDASen
dc.subjectBDCen
dc.subjectR2Cen
dc.subject.lccQDen
dc.title2′-alkynylnucleotides : a sequence- and spin label-flexible strategy for EPR Spectroscopy in DNAen
dc.typeJournal articleen
dc.contributor.sponsorEPSRCen
dc.contributor.sponsorThe Royal Societyen
dc.contributor.sponsorThe Wellcome Trusten
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1021/jacs.6b05421
dc.description.statusPeer revieweden
dc.identifier.grantnumberEP/L022044/1en
dc.identifier.grantnumberUF090121en
dc.identifier.grantnumber099149/Z/12/Zen


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