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2′-alkynylnucleotides : a sequence- and spin label-flexible strategy for EPR Spectroscopy in DNA
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dc.contributor.author | Haugland, Marius M. | |
dc.contributor.author | El-Sagheer, Afaf H. | |
dc.contributor.author | Porter, Rachel J. | |
dc.contributor.author | Peña, Javier | |
dc.contributor.author | Brown, Tom | |
dc.contributor.author | Anderson, Edward A. | |
dc.contributor.author | Lovett, Janet E. | |
dc.date.accessioned | 2016-08-23T14:30:24Z | |
dc.date.available | 2016-08-23T14:30:24Z | |
dc.date.issued | 2016-07-27 | |
dc.identifier | 245029241 | |
dc.identifier | 1378c09e-52ea-4146-8ece-5d4be0c4a2cb | |
dc.identifier | 84979955517 | |
dc.identifier | 000380730000020 | |
dc.identifier.citation | Haugland , M M , El-Sagheer , A H , Porter , R J , Peña , J , Brown , T , Anderson , E A & Lovett , J E 2016 , ' 2′-alkynylnucleotides : a sequence- and spin label-flexible strategy for EPR Spectroscopy in DNA ' , Journal of the American Chemical Society , vol. 138 , no. 29 , pp. 9069-9072 . https://doi.org/10.1021/jacs.6b05421 | en |
dc.identifier.issn | 0002-7863 | |
dc.identifier.other | ORCID: /0000-0002-3561-450X/work/40301303 | |
dc.identifier.uri | https://hdl.handle.net/10023/9364 | |
dc.description | TB and AHE-S thank the BBSRC (sLOLA grant BB/J001694/1). EAA thanks the EPSRC (EP/K005391/1, EP/M019195/1). JEL thanks the EPSRC (EP/LO22044/1), and the Royal Society for a University Research Fellowship and grant RF120645. We thank the Wellcome Trust for the Q-band EPR spectrometer (099149/Z/12/Z), the EPSRC National EPR Service for spectrometer time, and Dr Hassane El Mkami for technical assistance. | en |
dc.description.abstract | Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2′-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron-electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information. | |
dc.format.extent | 4 | |
dc.format.extent | 1421071 | |
dc.language.iso | eng | |
dc.relation.ispartof | Journal of the American Chemical Society | en |
dc.subject | QD Chemistry | en |
dc.subject | Biochemistry | en |
dc.subject | Catalysis | en |
dc.subject | Colloid and Surface Chemistry | en |
dc.subject | Chemistry(all) | en |
dc.subject | NDAS | en |
dc.subject | BDC | en |
dc.subject | R2C | en |
dc.subject.lcc | QD | en |
dc.title | 2′-alkynylnucleotides : a sequence- and spin label-flexible strategy for EPR Spectroscopy in DNA | en |
dc.type | Journal article | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | The Royal Society | en |
dc.contributor.sponsor | The Wellcome Trust | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | https://doi.org/10.1021/jacs.6b05421 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | EP/L022044/1 | en |
dc.identifier.grantnumber | UF090121 | en |
dc.identifier.grantnumber | 099149/Z/12/Z | en |
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