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dc.contributor.authorLuke, Garry Alec
dc.contributor.authorRyan, Martin Denis
dc.date.accessioned2014-09-30T23:01:37Z
dc.date.available2014-09-30T23:01:37Z
dc.date.issued2013-10
dc.identifier71028375
dc.identifier87f6131f-c4cc-4136-9da1-ca97f2c98821
dc.identifier84884224657
dc.identifier.citationLuke , G A & Ryan , M D 2013 , ' The protein coexpression problem in biotechnology and biomedicine : virus 2A and 2A-like sequences provide a solution ' , Future Virology , vol. 8 , no. 10 , pp. 983-996 . https://doi.org/10.2217/fvl.13.82en
dc.identifier.issn1746-0794
dc.identifier.otherORCID: /0000-0002-0012-0614/work/47136059
dc.identifier.urihttps://hdl.handle.net/10023/5518
dc.descriptionThe authors acknowledge the support of the UK Biotechnology and Biological Sciences Research Council (BBSRC), the Wellcome Trust and the UK Medical Research Council (MRC).en
dc.description.abstractSynthetic biology enables us to create genes virtually at will. Ensuring that multiple genes are efficiently co-expressed within the same cell – to assemble multimeric complexes, to transfer biochemical pathways, to transfer ‘traits’, is more problematic. Viruses such as picornaviruses accomplish exactly this task: they generate multiple, different, proteins from a single open reading frame. The study of how foot-and-mouth disease virus (FMDV) controls it’s protein biogenesis lead to the discovery of a short oligopeptide sequence, ‘2A’, that is able to mediate a co-translational ‘cleavage’ between proteins. 2A and ‘2A-like’ sequences (from other viruses and cellular sequences) can be used to concatenate multiple gene sequences into a single gene, ensuring their co-expression within the same cell. These sequences are now being used in the treatment of cancer, in the production of pluripotent stem cells, to create transgenic plants and animals amongst a host of other biotechnological and biomedical applications.
dc.format.extent14
dc.format.extent615574
dc.language.isoeng
dc.relation.ispartofFuture Virologyen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.titleThe protein coexpression problem in biotechnology and biomedicine : virus 2A and 2A-like sequences provide a solutionen
dc.typeJournal articleen
dc.contributor.sponsorBBSRCen
dc.contributor.sponsorMedical Research Councilen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doi10.2217/fvl.13.82
dc.description.statusPeer revieweden
dc.date.embargoedUntil2014-10-01
dc.identifier.urlhttp://www.futuremedicine.com/loi/fvlen
dc.identifier.grantnumberBB/H007849/1en
dc.identifier.grantnumberG0901002en


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