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The protein coexpression problem in biotechnology and biomedicine : virus 2A and 2A-like sequences provide a solution

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Ryan_2013_FutVir_TheProtein.pdf (601.1Kb)
Date
10/2013
Author
Luke, Garry Alec
Ryan, Martin Denis
Funder
BBSRC
Medical Research Council
Grant ID
BB/H007849/1
G0901002
Keywords
SDG 3 - Good Health and Well-being
Metadata
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Abstract
Synthetic biology enables us to create genes virtually at will. Ensuring that multiple genes are efficiently co-expressed within the same cell – to assemble multimeric complexes, to transfer biochemical pathways, to transfer ‘traits’, is more problematic. Viruses such as picornaviruses accomplish exactly this task: they generate multiple, different, proteins from a single open reading frame. The study of how foot-and-mouth disease virus (FMDV) controls it’s protein biogenesis lead to the discovery of a short oligopeptide sequence, ‘2A’, that is able to mediate a co-translational ‘cleavage’ between proteins. 2A and ‘2A-like’ sequences (from other viruses and cellular sequences) can be used to concatenate multiple gene sequences into a single gene, ensuring their co-expression within the same cell. These sequences are now being used in the treatment of cancer, in the production of pluripotent stem cells, to create transgenic plants and animals amongst a host of other biotechnological and biomedical applications.
Citation
Luke , G A & Ryan , M D 2013 , ' The protein coexpression problem in biotechnology and biomedicine : virus 2A and 2A-like sequences provide a solution ' , Future Virology , vol. 8 , no. 10 , pp. 983-996 . https://doi.org/10.2217/fvl.13.82
Publication
Future Virology
Status
Peer reviewed
DOI
https://doi.org/10.2217/fvl.13.82
ISSN
1746-0794
Type
Journal article
Rights
Copyright, Future Medicine Ltd.
Description
The authors acknowledge the support of the UK Biotechnology and Biological Sciences Research Council (BBSRC), the Wellcome Trust and the UK Medical Research Council (MRC).
Collections
  • Biology Research
  • Biomedical Sciences Research Complex (BSRC) Research
  • University of St Andrews Research
URL
http://www.futuremedicine.com/loi/fvl
URI
http://hdl.handle.net/10023/5518

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