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dc.contributor.authorPerni, S.
dc.contributor.authorHackett, L.
dc.contributor.authorGoss, R.J.M.
dc.contributor.authorSimmons, M.J.
dc.contributor.authorOverton, T.W.
dc.date.accessioned2014-08-26T16:01:00Z
dc.date.available2014-08-26T16:01:00Z
dc.date.issued2013-11-04
dc.identifier.citationPerni , S , Hackett , L , Goss , R J M , Simmons , M J & Overton , T W 2013 , ' Optimisation of engineered Escherichia coli biofilms for enzymatic biosynthesis of L-halotryptophans ' AMB Express , vol. 3 , pp. 1-10 . DOI: 10.1186/2191-0855-3-66en
dc.identifier.issn2191-0855
dc.identifier.otherPURE: 143502194
dc.identifier.otherPURE UUID: 43c28864-5e57-4027-92b4-d0a16dec383f
dc.identifier.otherScopus: 84891446993
dc.identifier.urihttp://hdl.handle.net/10023/5241
dc.descriptionThis study was funded by a UK Biotechnology & Biological Sciences Research Council grant (BB/I006834/1) to MJS, RJMG and TWO and a quota PhD studentship to LH. The Accuri C6 instrument was awarded to TWO as a BD Accuri Creativity Award.en
dc.description.abstractEngineered biofilms comprising a single recombinant species have demonstrated remarkable activity as novel biocatalysts for a range of applications. In this work, we focused on the biotransformation of 5-haloindole into 5-halotryptophan, a pharmaceutical intermediate, using Escherichia coli expressing a recombinant tryptophan synthase enzyme encoded by plasmid pSTB7. To optimise the reaction we compared two E. coli K-12 strains (MC4100 and MG1655) and their ompR234 mutants, which overproduce the adhesin curli (PHL644 and PHL628). The ompR234 mutation increased the quantity of biofilm in both MG1655 and MC4100 backgrounds. In all cases, no conversion of 5-haloindoles was observed using cells without the pSTB7 plasmid. Engineered biofilms of strains PHL628 pSTB7 and PHL644 pSTB7 generated more 5-halotryptophan than their corresponding planktonic cells. Flow cytometry revealed that the vast majority of cells were alive after 24 hour biotransformation reactions, both in planktonic and biofilm forms, suggesting that cell viability was not a major factor in the greater performance of biofilm reactions. Monitoring 5-haloindole depletion, 5-halotryptophan synthesis and the percentage conversion of the biotransformation reaction suggested that there were inherent differences between strains MG1655 and MC4100, and between planktonic and biofilm cells, in terms of tryptophan and indole metabolism and transport. The study has reinforced the need to thoroughly investigate bacterial physiology and make informed strain selections when developing biotransformation reactions.en
dc.format.extent10en
dc.language.isoeng
dc.relation.ispartofAMB Expressen
dc.rights© 2013 Perni et al.; licensee Springer. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectE. colien
dc.subjectBiofilmen
dc.subjectBiotransformationen
dc.subjectHaloindoleen
dc.subjectHalotryptophanen
dc.subjectQD Chemistryen
dc.subject.lccQDen
dc.titleOptimisation of engineered Escherichia coli biofilms for enzymatic biosynthesis of L-halotryptophansen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Chemistryen
dc.contributor.institutionUniversity of St Andrews. EaSTCHEMen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1186/2191-0855-3-66
dc.description.statusPeer revieweden
dc.identifier.urlhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84891446993&partnerID=8YFLogxK


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