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Crystallization of Ranasmurfin, a blue coloured protein from Polypedates leucomystax

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ActaCrystF2006_62_11_McMahon.pdf (284.7Kb)
Date
11/2006
Author
McMahon, Stephen
Walsh, MA
Ching, RTY
Carter, Lester
Dorward, M
Johnson, Kenneth Alan
Liu, Huanting
Oke, Muse
Block Jr, C
Kennedy, MW
Latiff, AA
Cooper, A
Taylor, Garry Lindsay
White, Malcolm Frederick
Naismith, James Henderson
Funder
BBSRC
Grant ID
BBS/B/14426
Keywords
QH426 Genetics
Metadata
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Abstract
Ranasmurfin, a previously uncharacterized similar to 13 kDa blue protein found in the nests of the frog Polypedates leucomystax, has been purified and crystallized. The crystals are an intense blue colour and diffract to 1.51 angstrom with P2(1) symmetry and unit-cell parameters a = 40.9, b = 59.9, c = 45.0 angstrom, beta = 93.3 degrees. Self-rotation function analysis indicates the presence of a dimer in the asymmetric unit. Biochemical data suggest that the blue colour of the protein is related to dimer formation. Sequence data for the protein are incomplete, but thus far have identified no model for molecular replacement. A fluorescence scan shows a peak at 9.676 keV, indicating that the protein binds zinc and suggesting a route for structure solution.
Citation
McMahon , S , Walsh , MA , Ching , RTY , Carter , L , Dorward , M , Johnson , K A , Liu , H , Oke , M , Block Jr , C , Kennedy , MW , Latiff , AA , Cooper , A , Taylor , G L , White , M F & Naismith , J H 2006 , ' Crystallization of Ranasmurfin, a blue coloured protein from Polypedates leucomystax ' , Acta Crystallographica. Section F, Structural biology and crystallization communications , vol. 62 , no. Pt 11 , pp. 1124-1126 . https://doi.org/10.1107/S1744309106040036
Publication
Acta Crystallographica. Section F, Structural biology and crystallization communications
Status
Peer reviewed
DOI
https://doi.org/10.1107/S1744309106040036
ISSN
1744-3091
Type
Journal article
Rights
Copyright © 2006 International Union of Crystallography
Collections
  • University of St Andrews Research
URL
http://www.scopus.com/inward/record.url?scp=33750576063&partnerID=8YFLogxK
http://ukpmc.ac.uk/abstract/MED/17077494
URI
http://hdl.handle.net/10023/3437

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