Now showing items 1-8 of 8

    • Characterisation of a Holliday junction-resolving enzyme from Schizosaccharomyces pombe 

      White, Malcolm Frederick; Lilley, DMJ (1997-11) - Journal article
      The rearrangement and repair of DNA by homologous recombination involves the creation of Holliday junctions, which are cleaved by a class of junction-specific endonucleases to generate recombinant duplex DNA products, Only ...
    • Crystallization of Ranasmurfin, a blue coloured protein from Polypedates leucomystax 

      McMahon, Stephen; Walsh, MA; Ching, RTY; Carter, Lester; Dorward, M; Johnson, Kenneth Alan; Liu, Huanting; Oke, Muse; Block Jr, C; Kennedy, MW; Latiff, AA; Cooper, A; Taylor, Garry Lindsay; White, Malcolm Frederick; Naismith, James Henderson (2006-11) - Journal article
      Ranasmurfin, a previously uncharacterized similar to 13 kDa blue protein found in the nests of the frog Polypedates leucomystax, has been purified and crystallized. The crystals are an intense blue colour and diffract to ...
    • DNA repair in the Archaea – an emerging picture 

      White, Malcolm Frederick; Allers, Thorsten (2018-07) - Journal item
      There has long been a fascination in the DNA Repair pathways of archaea, for two main reasons. Firstly, many archaea inhabit extreme environments where the rate of physical damage to DNA is accelerated. These archaea might ...
    • DNA‐Interacting characteristics of the archaeal Rudiviral protein SIRV2_Gp1 

      Peeters, Eveline; Boon, Maarten; Rollie, Clare Jane Catherine; Willaert, Ronnie G.; Voet, Marleen; White, Malcolm Frederick; Prangishvili, David; Lavigne, Rob; Quax, Tessa E. F. (2017-07) - Journal article
      Whereas the infection cycles of many bacterial and eukaryotic viruses have been characterized in detail, those of archaeal viruses remain largely unexplored. Recently, studies on a few model archaeal viruses such as SIRV2 ...
    • High-affinity RNA binding by a hyperthermophilic single-stranded DNA-binding protein 

      Morten, Michael John; Gamsjaeger, Roland; Cubeddu, Liza; Kariawasam, Ruvini; Peregrina, Jose Ramon; Penedo-Esteiro, Juan Carlos; White, Malcolm Frederick (2017-03) - Journal article
      Single-stranded DNA-binding proteins (SSBs), including replication protein A (RPA) in eukaryotes, play a central role in DNA replication, recombination, and repair. SSBs utilise an oligonucleotide/oligosaccharide-binding ...
    • Multiple nucleic acid cleavage modes in divergent type III CRISPR systems 

      Zhang, Jing; Graham, Shirley; Tello, Agnes; Liu, Huanting; White, Malcolm Frederick (2016-02-29) - Journal article
      CRISPR-Cas is an RNA-guided adaptive immune system that protects bacteria and archaea from invading nucleic acids. Type III systems (Cmr, Csm) have been shown to cleave RNA targets in vitro and some are capable of ...
    • The Sulfolobus solfataricus radA paralogue sso0777 is DNA damage inducible and positively regulated by the Sta1 protein 

      Abella, M; Rodriquez, S; Paytubi, S; Campoy, S; White, Malcolm Frederick; Barbe, J (2007-11) - Journal article
      Little is known about the regulation of the DNA damage-mediated gene expression in archaea. Here we report that the addition of actinomycin D to Sulfolobus solfataricus cultures triggers the expression of the radA paralogue ...
    • A type III-B CRISPR–Cas effector complex mediating massive target DNA destruction 

      Han, Wenyuan; Li, Yingjun; Deng, Ling; Feng, Mingxia; Peng, Wenfang; Hallstrøm, Søren; Zhang, Jing; Peng, Nan; Liang, Yun Xiang; White, Malcolm Frederick; She, Qunxin (2017-02-28) - Journal article
      The CRISPR (clustered regularly interspaced short palindromic repeats) system protects archaea and bacteria by eliminating nucleic acid invaders in a crRNA-guided manner. The Sulfolobus islandicus type III-B effector Cmr-α ...