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dc.contributor.authorZhou, Linna
dc.contributor.authorIshizaki, Hironori
dc.contributor.authorSpitzer, Michaela
dc.contributor.authorTaylor, Kerrie
dc.contributor.authorTemperley, Nicholas
dc.contributor.authorJohnson, Stephen
dc.contributor.authorBrear, Paul
dc.contributor.authorGautier, Philippe
dc.contributor.authorZeng, Zhiqiang
dc.contributor.authorMitchell, Amy
dc.contributor.authorNarayan, Vikram
dc.contributor.authorMcNeil, Ewan
dc.contributor.authorMelton, David
dc.contributor.authorSmith, Terry K
dc.contributor.authorTyers, Mike
dc.contributor.authorWestwood, Nicholas James
dc.contributor.authorPatton, Elizabeth
dc.date.accessioned2012-08-14T14:31:01Z
dc.date.available2012-08-14T14:31:01Z
dc.date.issued2012-07-27
dc.identifier.citationZhou , L , Ishizaki , H , Spitzer , M , Taylor , K , Temperley , N , Johnson , S , Brear , P , Gautier , P , Zeng , Z , Mitchell , A , Narayan , V , McNeil , E , Melton , D , Smith , T K , Tyers , M , Westwood , N J & Patton , E 2012 , ' ALDH2 mediates 5-nitrofuran activity in multiple species ' , Chemistry and Biology , vol. 19 , no. 7 , pp. 883-892 . https://doi.org/10.1016/j.chembiol.2012.05.017en
dc.identifier.issn1074-5521
dc.identifier.otherPURE: 22872483
dc.identifier.otherPURE UUID: dd308072-1db7-46f2-b348-fccdce427740
dc.identifier.otherPubMed: 22840776
dc.identifier.otherWOS: 000307261100014
dc.identifier.otherScopus: 84864408346
dc.identifier.otherORCID: /0000-0003-0630-0138/work/56424151
dc.identifier.urihttp://hdl.handle.net/10023/3043
dc.description.abstractUnderstanding how drugs work in vivo is critical for drug design and for maximizing the potential of currently available drugs. 5-nitrofurans are a class of pro-drugs widely used to treat bacterial and trypanosome infections, but despite relative specificity 5-nitrofurans often cause serious toxic side-effects in people. Here, we use yeast, zebrafish and human in vitro systems to assess the biological activity of 5-nitrofurans, and identify a conserved interaction between aldehyde dehydrogenase (ALDH) 2 and 5-nitrofurans across these species. In addition, we show that the activity of nifurtimox, a 5-nitrofuran anti-trypanosome pro-drug, is dependent on zebrafish Aldh2 and that nifurtimox is a substrate for human ALDH2. This study reveals a conserved and biologically relevant ALDH2-5-nitrofuran interaction that may have important implications for managing the toxicity of 5nitrofuran treatment.
dc.format.extent10
dc.language.isoeng
dc.relation.ispartofChemistry and Biologyen
dc.rightsThis is an author version of this article. The published version Copyright © 2012 Elsevier Ltd is available at www.sciencedirect.comen
dc.subjectQD Chemistryen
dc.subject.lccQDen
dc.titleALDH2 mediates 5-nitrofuran activity in multiple speciesen
dc.typeJournal articleen
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews.School of Chemistryen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.EaSTCHEMen
dc.identifier.doihttps://doi.org/10.1016/j.chembiol.2012.05.017
dc.description.statusPeer revieweden


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