Uncovering the chemistry of C-C bond formation in C-nucleoside biosynthesis : crystal structure of a C -glycoside synthase/PRPP complex
Abstract
The enzyme ForT catalyzes C–C bond formation between 5′-phosphoribosyl-1′-pyrophosphate (PRPP) and 4-amino-1H-pyrazole-3,5-dicarboxylate to make a key intermediate in the biosynthesis of formycin A 5′-phosphate by Streptomyces kaniharaensis. We report the 2.5 Å resolution structure of the ForT/PRPP complex and locate active site residues critical for PRPP recognition and catalysis.
Citation
Gao , S , Radadiya , A , Li , W , Liu , H , Zhu , W , De Crécy-Lagard , V , Richards , N G J & Naismith , J H 2020 , ' Uncovering the chemistry of C-C bond formation in C-nucleoside biosynthesis : crystal structure of a C -glycoside synthase/PRPP complex ' , Chemical Communications , vol. 56 , no. 55 , pp. 7617-7620 . https://doi.org/10.1039/d0cc02834g
Publication
Chemical Communications
Status
Peer reviewed
ISSN
1359-7345Type
Journal article
Rights
Copyright © 2020 The Author(s). Open Access Article. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Description
Authors thank the Diamond Light Source for beam time allocation and beam line staff for assistance with data collection. Funding for these studies was provided by BBSRC (BB/T006161/1 & BB/T006188/1 to J. H. N. & N. G. J. R., respectively), and the National Institutes of Health (R01 GM129793 to V. d. C.-L.)Collections
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