Effective high-throughput blood pooling strategy before DNA extraction for detection of malaria in low-transmission settings
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In the era of (pre) elimination setting, the prevalence of malaria has been decreasing in most of the previously endemic areas. Therefore, effective cost-and time-saving validated pooling strategy is needed for detection of malaria in low transmission settings. In this study, optimal pooling numbers and lowest detection limit were assessed using known density samples prepared systematically, followed by genomic DNA extraction and nested PCR. Pooling strategy that composed of 10 samples in 1 pool, 20 μl in 1 sample, was optimal, and the parasite density as low as 2 p/μl for both falciparum and vivax infection was enough for detection of malaria. This pooling method showed effectiveness for handling of a huge number of samples in low transmission settings.
Nyunt , M H , Kyaw , M P , Thant , K Z , Shein , T , Han , S S , Zaw , N N , Han , J-H , Lee , S-K , Muh , F , Kim , J-Y , Cho , S-H , Lee , S-E , Yang , E-J , Chang , C L & Han , E-T 2016 , ' Effective high-throughput blood pooling strategy before DNA extraction for detection of malaria in low-transmission settings ' Korean Journal of Parasitology , vol. 54 , no. 3 , pp. 253-259 . https://doi.org/10.3347/kjp.2016.54.3.253
Korean Journal of Parasitology
© 2016, Korean Society for Parasitology and Tropical Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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