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dc.contributor.authorNyunt, Myat Htut
dc.contributor.authorKyaw, Myat Phone
dc.contributor.authorThant, Kyaw Zin
dc.contributor.authorShein, Thinzer
dc.contributor.authorHan, Soe Soe
dc.contributor.authorZaw, Ni Ni
dc.contributor.authorHan, Jin-Hee
dc.contributor.authorLee, Seong-Kyun
dc.contributor.authorMuh, Fauzi
dc.contributor.authorKim, Jung-Yeon
dc.contributor.authorCho, Shin-Hyeong
dc.contributor.authorLee, Sang-Eun
dc.contributor.authorYang, Eun-Jeong
dc.contributor.authorChang, Chulhun L.
dc.contributor.authorHan, Eun-Taek
dc.identifier.citationNyunt , M H , Kyaw , M P , Thant , K Z , Shein , T , Han , S S , Zaw , N N , Han , J-H , Lee , S-K , Muh , F , Kim , J-Y , Cho , S-H , Lee , S-E , Yang , E-J , Chang , C L & Han , E-T 2016 , ' Effective high-throughput blood pooling strategy before DNA extraction for detection of malaria in low-transmission settings ' , Korean Journal of Parasitology , vol. 54 , no. 3 , pp. 253-259 .
dc.identifier.otherORCID: /0000-0002-3966-9110/work/59698783
dc.description.abstractIn the era of (pre) elimination setting, the prevalence of malaria has been decreasing in most of the previously endemic areas. Therefore, effective cost-and time-saving validated pooling strategy is needed for detection of malaria in low transmission settings. In this study, optimal pooling numbers and lowest detection limit were assessed using known density samples prepared systematically, followed by genomic DNA extraction and nested PCR. Pooling strategy that composed of 10 samples in 1 pool, 20 μl in 1 sample, was optimal, and the parasite density as low as 2 p/μl for both falciparum and vivax infection was enough for detection of malaria. This pooling method showed effectiveness for handling of a huge number of samples in low transmission settings.
dc.relation.ispartofKorean Journal of Parasitologyen
dc.subjectPlasmodium falciparumen
dc.subjectPlasmodium vivaxen
dc.subjectPooling strategyen
dc.subjectLow-transmission settingen
dc.subjectNested PCRen
dc.subjectQR180 Immunologyen
dc.subjectRM Therapeutics. Pharmacologyen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.titleEffective high-throughput blood pooling strategy before DNA extraction for detection of malaria in low-transmission settingsen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.description.statusPeer revieweden

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