Probing the interaction of nanoparticles with small molecules in real time via quartz crystal microbalance monitoring
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Despite extensive advances in the field of molecular recognition, the real-time monitoring of small molecule binding to nanoparticles (NP) remains a challenge. To this end, we report on a versatile approach, based on quartz crystal microbalance with dissipation monitoring, for the stepwise in situ quantification of gold nanoparticle (AuNPs) immobilisation and subsequent uptake and release of binding partners. AuNPs stabilised by thiol-bound ligand shells of prescribed chemical composition were densely immobilised onto gold surfaces via dithiol linkers. The boronate ester formation between salicylic acid derivatives in solution and boronic acids in the AuNP ligand shell was then studied in real time, revealing a drastic effect of both ligand architecture and Lewis base concentration on the interaction strength. The binding kinetics were analysed with frequency response modelling for a thorough comparison of binding parameters including relaxation time as well as association rate constant. The results directly mirror those from previously reported in-depth studies using nuclear magnetic resonance spectroscopy. By achieving quantitative characterisation of selective binding of analytes with molecular weight below 300 Da, this new method enables rapid, low cost, rational screening of AuNP candidates for molecular recognition.
Yang , Y , Poss , G , Weng , Y , Qi , R , Zheng , H , Nianias , N , Kay , E R & Guldin , S 2019 , ' Probing the interaction of nanoparticles with small molecules in real time via quartz crystal microbalance monitoring ' , Nanoscale , vol. 11 , no. 23 , pp. 11107-11113 . https://doi.org/10.1039/C9NR03162F
Copyright © 2019 The Authors. Open Access Article. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
DescriptionY. Y. acknowledges University College London (UCL) for the Overseas Research Scholarship and the Graduate Research Scholarship. The project received funding from the European Unions Horizon 2020 research and innovation programme under grant agreement no. 633635 (DIACHEMO) and the EPSRC (grant number EP/J500549/1).
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