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dc.contributor.authorSuleiman, Suzanne
dc.contributor.authorSmith, Valerie J.
dc.contributor.authorDyrynda, Elisabeth A.
dc.date.accessioned2018-06-23T23:32:08Z
dc.date.available2018-06-23T23:32:08Z
dc.date.issued2017-11
dc.identifier.citationSuleiman , S , Smith , V J & Dyrynda , E A 2017 , ' Unusual tissue distribution of carcinin, an antibacterial crustin, in the crab, Carcinus maenas , reveals its multi-functionality ' , Developmental and Comparative Immunology , vol. 76 , pp. 274-284 . https://doi.org/10.1016/j.dci.2017.06.010en
dc.identifier.issn0145-305X
dc.identifier.otherPURE: 250334471
dc.identifier.otherPURE UUID: e28eda88-c8c5-4316-8768-d8f8aca4d4a8
dc.identifier.otherRIS: urn:DA0EEC2F05CFEEF0A70F3C5034656C2C
dc.identifier.otherScopus: 85021712851
dc.identifier.otherWOS: 000407985100030
dc.identifier.urihttp://hdl.handle.net/10023/14499
dc.descriptionFunding was provided by a scholarship to SS by the Syrian Ministry of Higher Education, administered by the British Council. The Alumni Fund of Heriot-Watt University and the British Council also provided additional financial support to SS.en
dc.description.abstractCrustins are whey acidic four-disulphide core (WFDSC) domain-containing proteins in decapods that are widely regarded as antimicrobial agents that contribute to host defence. Whilst there have been many analyses of crustin gene expression in tissues, few studies have been made of the distribution of the natural proteins. Here we report an immunostaining investigation of carcinin, a native crustin from Carcinus maenas, in the body organs. The results show that the protein is largely confined to the haemocytes with only a weak signal detected in the heart, hepatopancreas and midgut caecum where it is restricted to the outer surfaces. Importantly, carcinin was seen to be deposited by the haemocytes on these surfaces. Higher levels of staining were detected in the gonads with carcinin particularly abundant in the capsule of ovary as well as some oocytes. Conspicuous staining was further evident in the cuticle of the eyestalk peduncles. Ablation of the eyestalks resulted in a reduction of carcinin in the maturing ovary with the mature eggs rarely displaying a strong signal for the protein. Interestingly, the degree of carcinin also strongly increased in the healing peduncle, indicating that the protein may be associated with wounding, cell damage and/or tissue regeneration.
dc.language.isoeng
dc.relation.ispartofDevelopmental and Comparative Immunologyen
dc.rights© 2017 Elsevier Ltd. All rights reserved. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1016/j.dci.2017.06.010en
dc.subjectWFDSC-Domain-containing proteinen
dc.subjectAntimicrobial protein (AMP)en
dc.subjectHaemocytesen
dc.subjectOvaryen
dc.subjectEyestalk peduncleen
dc.subjectTissue repairen
dc.subjectQH301 Biologyen
dc.subjectQR180 Immunologyen
dc.subjectNDASen
dc.subject.lccQH301en
dc.subject.lccQR180en
dc.titleUnusual tissue distribution of carcinin, an antibacterial crustin, in the crab, Carcinus maenas, reveals its multi-functionalityen
dc.typeJournal articleen
dc.description.versionPostprinten
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Scottish Oceans Instituteen
dc.identifier.doihttps://doi.org/10.1016/j.dci.2017.06.010
dc.description.statusPeer revieweden
dc.date.embargoedUntil2018-06-23
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0145305X17302690#appd001en


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