St Andrews Research Repository

St Andrews University Home
View Item 
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  • Login
JavaScript is disabled for your browser. Some features of this site may not work without it.

Unusual tissue distribution of carcinin, an antibacterial crustin, in the crab, Carcinus maenas, reveals its multi-functionality

Thumbnail
View/Open
Smith_2017_DCI_Carcinin_SI_AAM.pdf (987.9Kb)
Smith_2017_DCI_Carcinin_AAM.pdf (38.83Mb)
Date
11/2017
Author
Suleiman, Suzanne
Smith, Valerie J.
Dyrynda, Elisabeth A.
Keywords
WFDSC-Domain-containing protein
Antimicrobial protein (AMP)
Haemocytes
Ovary
Eyestalk peduncle
Tissue repair
QH301 Biology
QR180 Immunology
NDAS
Metadata
Show full item record
Altmetrics Handle Statistics
Altmetrics DOI Statistics
Abstract
Crustins are whey acidic four-disulphide core (WFDSC) domain-containing proteins in decapods that are widely regarded as antimicrobial agents that contribute to host defence. Whilst there have been many analyses of crustin gene expression in tissues, few studies have been made of the distribution of the natural proteins. Here we report an immunostaining investigation of carcinin, a native crustin from Carcinus maenas, in the body organs. The results show that the protein is largely confined to the haemocytes with only a weak signal detected in the heart, hepatopancreas and midgut caecum where it is restricted to the outer surfaces. Importantly, carcinin was seen to be deposited by the haemocytes on these surfaces. Higher levels of staining were detected in the gonads with carcinin particularly abundant in the capsule of ovary as well as some oocytes. Conspicuous staining was further evident in the cuticle of the eyestalk peduncles. Ablation of the eyestalks resulted in a reduction of carcinin in the maturing ovary with the mature eggs rarely displaying a strong signal for the protein. Interestingly, the degree of carcinin also strongly increased in the healing peduncle, indicating that the protein may be associated with wounding, cell damage and/or tissue regeneration.
Citation
Suleiman , S , Smith , V J & Dyrynda , E A 2017 , ' Unusual tissue distribution of carcinin, an antibacterial crustin, in the crab, Carcinus maenas , reveals its multi-functionality ' , Developmental and Comparative Immunology , vol. 76 , pp. 274-284 . https://doi.org/10.1016/j.dci.2017.06.010
Publication
Developmental and Comparative Immunology
Status
Peer reviewed
DOI
https://doi.org/10.1016/j.dci.2017.06.010
ISSN
0145-305X
Type
Journal article
Rights
© 2017 Elsevier Ltd. All rights reserved. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1016/j.dci.2017.06.010
Description
Funding was provided by a scholarship to SS by the Syrian Ministry of Higher Education, administered by the British Council. The Alumni Fund of Heriot-Watt University and the British Council also provided additional financial support to SS.
Collections
  • University of St Andrews Research
URL
http://www.sciencedirect.com/science/article/pii/S0145305X17302690#appd001
URI
http://hdl.handle.net/10023/14499

Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

Advanced Search

Browse

All of RepositoryCommunities & CollectionsBy Issue DateNamesTitlesSubjectsClassificationTypeFunderThis CollectionBy Issue DateNamesTitlesSubjectsClassificationTypeFunder

My Account

Login

Open Access

To find out how you can benefit from open access to research, see our library web pages and Open Access blog. For open access help contact: openaccess@st-andrews.ac.uk.

Accessibility

Read our Accessibility statement.

How to submit research papers

The full text of research papers can be submitted to the repository via Pure, the University's research information system. For help see our guide: How to deposit in Pure.

Electronic thesis deposit

Help with deposit.

Repository help

For repository help contact: Digital-Repository@st-andrews.ac.uk.

Give Feedback

Cookie policy

This site may use cookies. Please see Terms and Conditions.

Usage statistics

COUNTER-compliant statistics on downloads from the repository are available from the IRUS-UK Service. Contact us for information.

© University of St Andrews Library

University of St Andrews is a charity registered in Scotland, No SC013532.

  • Facebook
  • Twitter