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17ß-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats

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Date
05/05/2017
Author
Zafirovic, Sonja
Obradovic, Milan
Sudar-Milovanovic, Emina
Jovanovic, Aleksandra
Stanimirovic, Julijana
Stewart, Alan J.
Pitt, Samantha J.
Isenovic, Esma R.
Keywords
Estradiol
Obesity
Myocardial metabolism
Cardiovascular diseases
Inducible nitric oxide synthase
QH301 Biology
QH426 Genetics
NDAS
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Abstract
The aim of this study was to investigate the in vivo effects of estradiol (E2) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. Male Wistar rats were fed with a normal or a high fat (HF) diet (42% fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E2 while the other half were placebo-treated. 24h after treatment animals were sacrificed. E2 reduced cardiac free fatty acid (FFA) (p<0.05), L-arginine (p<0.01), iNOS mRNA (p<0.01), and protein (p<0.05) levels and translocation of the FFA transporter (CD36) (p<0.01) to the plasma membrane (PM) in HF fed rats. In contrast, Akt phosphorylation at Thr308 (p<0.05) and translocation of the glucose transporter GLUT4 (p<0.05) to the PM increased after E2 tretment in HF rats. Our results indicate that E2 acts via PI3K/Akt signaling pathway to partially protect myocardial metabolism by attenuating the detrimental effects of increased iNOS expression/activity in HF fed rats.
Citation
Zafirovic , S , Obradovic , M , Sudar-Milovanovic , E , Jovanovic , A , Stanimirovic , J , Stewart , A J , Pitt , S J & Isenovic , E R 2017 , ' 17ß-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats ' , Molecular and Cellular Endocrinology , vol. 446 , pp. 12-20 . https://doi.org/10.1016/j.mce.2017.02.001
Publication
Molecular and Cellular Endocrinology
Status
Peer reviewed
DOI
https://doi.org/10.1016/j.mce.2017.02.001
ISSN
0303-7207
Type
Journal article
Rights
Copyright © 2017, Elsevier Ltd. This work is made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1016/j.mce.2017.02.001
Description
This work is supported by the grant No.173033 (to E.R.I.) from the Ministry of Education, Science and Technological Development, Republic of Serbia.
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  • University of St Andrews Research
URL
http://www.sciencedirect.com/science/article/pii/S0303720717300771
URI
http://hdl.handle.net/10023/12661

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