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17ß-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats
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dc.contributor.author | Zafirovic, Sonja | |
dc.contributor.author | Obradovic, Milan | |
dc.contributor.author | Sudar-Milovanovic, Emina | |
dc.contributor.author | Jovanovic, Aleksandra | |
dc.contributor.author | Stanimirovic, Julijana | |
dc.contributor.author | Stewart, Alan J. | |
dc.contributor.author | Pitt, Samantha J. | |
dc.contributor.author | Isenovic, Esma R. | |
dc.date.accessioned | 2018-02-03T00:31:38Z | |
dc.date.available | 2018-02-03T00:31:38Z | |
dc.date.issued | 2017-05-05 | |
dc.identifier | 249051761 | |
dc.identifier | 39cf3c6c-e256-400b-af56-2299711c774f | |
dc.identifier | 85012867945 | |
dc.identifier | 000399509600002 | |
dc.identifier.citation | Zafirovic , S , Obradovic , M , Sudar-Milovanovic , E , Jovanovic , A , Stanimirovic , J , Stewart , A J , Pitt , S J & Isenovic , E R 2017 , ' 17ß-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats ' , Molecular and Cellular Endocrinology , vol. 446 , pp. 12-20 . https://doi.org/10.1016/j.mce.2017.02.001 | en |
dc.identifier.issn | 0303-7207 | |
dc.identifier.other | ORCID: /0000-0003-4580-1840/work/60195790 | |
dc.identifier.other | ORCID: /0000-0003-2257-1595/work/60196224 | |
dc.identifier.uri | https://hdl.handle.net/10023/12661 | |
dc.description | This work is supported by the grant No.173033 (to E.R.I.) from the Ministry of Education, Science and Technological Development, Republic of Serbia. | en |
dc.description.abstract | The aim of this study was to investigate the in vivo effects of estradiol (E2) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. Male Wistar rats were fed with a normal or a high fat (HF) diet (42% fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E2 while the other half were placebo-treated. 24h after treatment animals were sacrificed. E2 reduced cardiac free fatty acid (FFA) (p<0.05), L-arginine (p<0.01), iNOS mRNA (p<0.01), and protein (p<0.05) levels and translocation of the FFA transporter (CD36) (p<0.01) to the plasma membrane (PM) in HF fed rats. In contrast, Akt phosphorylation at Thr308 (p<0.05) and translocation of the glucose transporter GLUT4 (p<0.05) to the PM increased after E2 tretment in HF rats. Our results indicate that E2 acts via PI3K/Akt signaling pathway to partially protect myocardial metabolism by attenuating the detrimental effects of increased iNOS expression/activity in HF fed rats. | |
dc.format.extent | 9 | |
dc.format.extent | 1454850 | |
dc.language.iso | eng | |
dc.relation.ispartof | Molecular and Cellular Endocrinology | en |
dc.subject | Estradiol | en |
dc.subject | Obesity | en |
dc.subject | Myocardial metabolism | en |
dc.subject | Cardiovascular diseases | en |
dc.subject | Inducible nitric oxide synthase | en |
dc.subject | QH301 Biology | en |
dc.subject | QH426 Genetics | en |
dc.subject | NDAS | en |
dc.subject | SDG 3 - Good Health and Well-being | en |
dc.subject.lcc | QH301 | en |
dc.subject.lcc | QH426 | en |
dc.title | 17ß-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. Institute of Behavioural and Neural Sciences | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. Cellular Medicine Division | en |
dc.identifier.doi | https://doi.org/10.1016/j.mce.2017.02.001 | |
dc.description.status | Peer reviewed | en |
dc.date.embargoedUntil | 2018-02-02 | |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0303720717300771 | en |
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