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dc.contributor.authorZafirovic, Sonja
dc.contributor.authorObradovic, Milan
dc.contributor.authorSudar-Milovanovic, Emina
dc.contributor.authorJovanovic, Aleksandra
dc.contributor.authorStanimirovic, Julijana
dc.contributor.authorStewart, Alan J.
dc.contributor.authorPitt, Samantha J.
dc.contributor.authorIsenovic, Esma R.
dc.date.accessioned2018-02-03T00:31:38Z
dc.date.available2018-02-03T00:31:38Z
dc.date.issued2017-05-05
dc.identifier.citationZafirovic , S , Obradovic , M , Sudar-Milovanovic , E , Jovanovic , A , Stanimirovic , J , Stewart , A J , Pitt , S J & Isenovic , E R 2017 , ' 17ß-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats ' Molecular and Cellular Endocrinology , vol. 446 , pp. 12-20 . https://doi.org/10.1016/j.mce.2017.02.001en
dc.identifier.issn0303-7207
dc.identifier.otherPURE: 249051761
dc.identifier.otherPURE UUID: 39cf3c6c-e256-400b-af56-2299711c774f
dc.identifier.otherScopus: 85012867945
dc.identifier.urihttp://hdl.handle.net/10023/12661
dc.descriptionThis work is supported by the grant No.173033 (to E.R.I.) from the Ministry of Education, Science and Technological Development, Republic of Serbia.en
dc.description.abstractThe aim of this study was to investigate the in vivo effects of estradiol (E2) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. Male Wistar rats were fed with a normal or a high fat (HF) diet (42% fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E2 while the other half were placebo-treated. 24h after treatment animals were sacrificed. E2 reduced cardiac free fatty acid (FFA) (p<0.05), L-arginine (p<0.01), iNOS mRNA (p<0.01), and protein (p<0.05) levels and translocation of the FFA transporter (CD36) (p<0.01) to the plasma membrane (PM) in HF fed rats. In contrast, Akt phosphorylation at Thr308 (p<0.05) and translocation of the glucose transporter GLUT4 (p<0.05) to the PM increased after E2 tretment in HF rats. Our results indicate that E2 acts via PI3K/Akt signaling pathway to partially protect myocardial metabolism by attenuating the detrimental effects of increased iNOS expression/activity in HF fed rats.
dc.format.extent9
dc.language.isoeng
dc.relation.ispartofMolecular and Cellular Endocrinologyen
dc.rightsCopyright © 2017, Elsevier Ltd. This work is made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1016/j.mce.2017.02.001en
dc.subjectEstradiolen
dc.subjectObesityen
dc.subjectMyocardial metabolismen
dc.subjectCardiovascular diseasesen
dc.subjectInducible nitric oxide synthaseen
dc.subjectQH301 Biologyen
dc.subjectQH426 Geneticsen
dc.subjectNDASen
dc.subject.lccQH301en
dc.subject.lccQH426en
dc.title17ß-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in ratsen
dc.typeJournal articleen
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.Cellular Medicine Divisionen
dc.identifier.doihttps://doi.org/10.1016/j.mce.2017.02.001
dc.description.statusPeer revieweden
dc.date.embargoedUntil2018-02-02
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0303720717300771en


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