Haem-iron plays a key role in the regulation of the Ess/type VII secretion system of Staphylococcus aureus RN6390
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The Staphylococcus aureus type VII protein secretion system (T7SS) plays important roles in virulence and intra-species competition. Here we show that the T7SS in strain RN6390 is activated by supplementing the growth medium with haemoglobin, and its cofactor haemin (haem B). Transcript analysis and secretion assays suggest that activation by haemin occurs at a transcriptional and a post-translational level. Loss of T7 secretion activity by deletion of essC results in upregulation of genes required for iron acquisition. Taken together these findings suggest that the T7SS plays a role in iron homeostasis in at least some S. aureus strains.
Casabona , M G , Kneuper , H , Alferes de Lima , D , Harkins , C P , Zoltner , M , Hjerde , E , Holden , M T G & Palmer , T 2017 , ' Haem-iron plays a key role in the regulation of the Ess/type VII secretion system of Staphylococcus aureus RN6390 ' Microbiology , vol 163 , no. 12 , pp. 1839-1850 . DOI: 10.1099/mic.0.000579
© 2017 The Authors. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
DescriptionThis study was supported by the Wellcome Trust (through Investigator Award 10183/Z/15/Z to T. P. and through Clinical PhD studentship support to C. P. H. through grant 104241/z/14/z), the Biotechnology and Biological Sciences Research Council and the Medical Research Council (through grants BB/H007571/1 and MR/M011224/1, respectively).
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