Show simple item record

Files in this item

Thumbnail
Name:
Casabona_2017_Microbiology_Haem_iron_CC.pdf
Size:
1015.Kb
Format:
PDF
View/Open

Haem-iron plays a key role in the regulation of the Ess/type VII secretion system of Staphylococcus aureus RN6390

Item metadata

dc.contributor.authorCasabona, M Guillermina
dc.contributor.authorKneuper, Holger
dc.contributor.authorAlferes de Lima, Daniela
dc.contributor.authorHarkins, Catriona P
dc.contributor.authorZoltner, Martin
dc.contributor.authorHjerde, Erik
dc.contributor.authorHolden, Matthew T. G.
dc.contributor.authorPalmer, Tracy
dc.date.accessioned2017-11-30T14:30:07Z
dc.date.available2017-11-30T14:30:07Z
dc.date.issued2017-12-01
dc.identifier.citationCasabona , M G , Kneuper , H , Alferes de Lima , D , Harkins , C P , Zoltner , M , Hjerde , E , Holden , M T G & Palmer , T 2017 , ' Haem-iron plays a key role in the regulation of the Ess/type VII secretion system of Staphylococcus aureus RN6390 ' , Microbiology , vol. 163 , no. 12 , pp. 1839-1850 . https://doi.org/10.1099/mic.0.000579en
dc.identifier.issn1350-0872
dc.identifier.otherPURE: 251666266
dc.identifier.otherPURE UUID: 9a891453-b4ff-4323-b646-c792f52d13f4
dc.identifier.otherPubMed: 29171824
dc.identifier.otherScopus: 85044270463
dc.identifier.otherORCID: /0000-0002-4958-2166/work/60196386
dc.identifier.otherWOS: 000423293700011
dc.identifier.otherORCID: /0000-0002-5125-1657/work/75997034
dc.identifier.urihttps://hdl.handle.net/10023/12208
dc.descriptionThis study was supported by the Wellcome Trust (through Investigator Award 10183/Z/15/Z to T. P. and through Clinical PhD studentship support to C. P. H. through grant 104241/z/14/z), the Biotechnology and Biological Sciences Research Council and the Medical Research Council (through grants BB/H007571/1 and MR/M011224/1, respectively).en
dc.description.abstractThe Staphylococcus aureus type VII protein secretion system (T7SS) plays important roles in virulence and intra-species competition. Here we show that the T7SS in strain RN6390 is activated by supplementing the growth medium with haemoglobin, and its cofactor haemin (haem B). Transcript analysis and secretion assays suggest that activation by haemin occurs at a transcriptional and a post-translational level. Loss of T7 secretion activity by deletion of essC results in upregulation of genes required for iron acquisition. Taken together these findings suggest that the T7SS plays a role in iron homeostasis in at least some S. aureus strains.
dc.language.isoeng
dc.relation.ispartofMicrobiologyen
dc.rights© 2017 The Authors. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.en
dc.subjectStaphylococcus aureusen
dc.subjectProtein secretionen
dc.subjectIron homeostasisen
dc.subjectRNA-sequencingen
dc.subjectQH301 Biologyen
dc.subjectQR Microbiologyen
dc.subjectDASen
dc.subject.lccQH301en
dc.subject.lccQRen
dc.titleHaem-iron plays a key role in the regulation of the Ess/type VII secretion system of Staphylococcus aureus RN6390en
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Infection Groupen
dc.contributor.institutionUniversity of St Andrews. Infection and Global Health Divisionen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1099/mic.0.000579
dc.description.statusPeer revieweden


This item appears in the following Collection(s)

Show simple item record

Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.