St Andrews Research Repository

St Andrews University Home
View Item 
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  • Login
JavaScript is disabled for your browser. Some features of this site may not work without it.

Whole-genome sequencing to investigate a non-clonal melioidosis cluster on a remote Australian island

Thumbnail
View/Open
Holden_2017_MG_MelioidosisCluster_CC.pdf (230.4Kb)
Date
31/08/2017
Author
Sarovich, Derek S
Chapple, Stephanie N J
Price, Erin P
Mayo, Mark
Holden, Matthew T. G.
Peacock, Sharon J
Currie, Bart J
Keywords
Melioidosis
Source tracing
Burkholderia pseudomallei
Recombination
Population genetics
Outbreak
RA0421 Public health. Hygiene. Preventive Medicine
DAS
Metadata
Show full item record
Altmetrics Handle Statistics
Altmetrics DOI Statistics
Abstract
Melioidosis is a tropical disease caused by the bacterium Burkholderia pseudomallei. Outbreaks are uncommon and can generally be attributed to a single point source and strain. We used whole-genome sequencing to analyse B. pseudomallei isolates collected from an historical 2-year long case cluster that occurred in a remote northern Australian indigenous island community, where infections were previously linked to a contaminated communal water supply. We analysed the genome-wide relatedness of the two most common multilocus sequence types (STs) involved in the outbreak, STs 125 and 126. This analysis showed that although these STs were closely related on a whole-genome level, they demonstrated evidence of multiple recombination events that were unlikely to have occurred over the timeframe of the outbreak. Based on epidemiological and genetic data, we also identified two additional patients not previously associated with this outbreak. Our results confirm the previous hypothesis that a single unchlorinated water source harbouring multiple B. pseudomallei strains was linked to the outbreak, and that increased melioidosis risk in this community was associated with Piper methysticum root (kava) consumption.
Citation
Sarovich , D S , Chapple , S N J , Price , E P , Mayo , M , Holden , M T G , Peacock , S J & Currie , B J 2017 , ' Whole-genome sequencing to investigate a non-clonal melioidosis cluster on a remote Australian island ' , Microbial Genomics , vol. 3 , no. 8 , e000117 . https://doi.org/10.1099/mgen.0.000117
Publication
Microbial Genomics
Status
Peer reviewed
DOI
https://doi.org/10.1099/mgen.0.000117
ISSN
2057-5858
Type
Journal article
Rights
© 2017 The Authors. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
Description
This study was funded by National Health and Medical Research Council (Australia) grants 1046812 and 1098337. E. P. P. is in receipt of a University of the Sunshine Coast Research Fellowship.
Collections
  • University of St Andrews Research
URI
http://hdl.handle.net/10023/12075

Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

Advanced Search

Browse

All of RepositoryCommunities & CollectionsBy Issue DateNamesTitlesSubjectsClassificationTypeFunderThis CollectionBy Issue DateNamesTitlesSubjectsClassificationTypeFunder

My Account

Login

Open Access

To find out how you can benefit from open access to research, see our library web pages and Open Access blog. For open access help contact: openaccess@st-andrews.ac.uk.

Accessibility

Read our Accessibility statement.

How to submit research papers

The full text of research papers can be submitted to the repository via Pure, the University's research information system. For help see our guide: How to deposit in Pure.

Electronic thesis deposit

Help with deposit.

Repository help

For repository help contact: Digital-Repository@st-andrews.ac.uk.

Give Feedback

Cookie policy

This site may use cookies. Please see Terms and Conditions.

Usage statistics

COUNTER-compliant statistics on downloads from the repository are available from the IRUS-UK Service. Contact us for information.

© University of St Andrews Library

University of St Andrews is a charity registered in Scotland, No SC013532.

  • Facebook
  • Twitter