Now showing items 1-13 of 13

  • Building a genomic framework for prospective MRSA surveillance in the United Kingdom and the Republic of Ireland 

    Reuter, Sandra; Török, Estee M; Holden, Matthew Tg; Reynolds, Rosy; Raven, Kathy E; Blane, Beth; Donker, Tjibbe; Bentley, Stephen D; Aanensen, David M; Grundmann, Hajo; Feil, Edward J; Spratt, Brian G; Parkhill, Julian; Peacock, Sharon J (2016-02) - Journal article
    The correct interpretation of microbial sequencing data applied to surveillance and outbreak investigation depends on accessible genomic databases to provide vital genetic context. Our aim was to construct and describe a ...
  • Capturing the cloud of diversity reveals complexity and heterogeneity of MRSA carriage, infection and transmission 

    Paterson, Gavin K; Harrison, Ewan M; Murray, Gemma G R; Welch, John J; Warland, James H; Holden, Matthew T G; Morgan, Fiona J E; Ba, Xiaoliang; Koop, Gerrit; Harris, Simon R; Maskell, Duncan J; Peacock, Sharon J; Herrtage, Michael E; Parkhill, Julian; Holmes, Mark A (2015-03-27) - Journal article
    Genome sequencing is revolutionizing clinical microbiology and our understanding of infectious diseases. Previous studies have largely relied on the sequencing of a single isolate from each individual. However, it is not ...
  • Colony morphology variation of Burkholderia pseudomallei is associated with antigenic variation and O-polysaccharide modifications 

    Wikraiphat, Chanthiwa; Saiprom, Natnaree; Tandhavanant, Sarunporn; Heiss, Christian; Azadi, Parastoo; Wongsuvan, Gumphol; Tuanyok, Apichai; Holden, Matthew; Burtnick, Mary N; Brett, Paul J; Peacock, Sharon J; Chantratita, Narisara (2015-05) - Journal article
    Burkholderia pseudomallei is a CDC Tier 1 select agent that causes melioidosis, a severe disease in humans and animals. Persistent infections are common and there is currently no vaccine available. Lipopolysaccharide (LPS) ...
  • The Ess/Type VII secretion system of Staphylococcus aureus shows unexpected genetic diversity 

    Warne, Ben; Harkins, Catriona P; Harris, Simon R; Vatsiou, Alexandra; Stanley-Wall, Nicola; Parkhill, Julian; Peacock, Sharon J; Palmer, Tracy; Holden, Matthew T G (2016-03-13) - Journal article
    BACKGROUND: Type VII protein secretion (T7SS) is a specialised system for excreting extracellular proteins across bacterial cell membranes and has been associated with virulence in Staphylococcus aureus. The genetic diversity ...
  • Evolutionary dynamics of methicillin-resistant Staphylococcus aureus within a healthcare system 

    Hsu, Li-Yang; Harris, Simon R; Chlebowicz, Monika A; Lindsay, Jodi A; Koh, Tse-Hsien; Krishnan, Prabha; Tan, Thean-Yen; Hon, Pei-Yun; Grubb, Warren B; Bentley, Stephen D; Parkhill, Julian; Peacock, Sharon J; Holden, Matthew Tg (2015-04-23) - Journal article
    Background In the past decade, several countries have seen gradual replacement of endemic multi-resistant healthcare-associated methicillin-resistant Staphylococcus aureus (MRSA) with clones that are more susceptible to ...
  • Evolutionary trade-offs underlie the multi-faceted virulence of Staphylococcus aureus 

    Laabei, Maisem; Uhlemann, Anne-Catrin; Lowy, Franklin D; Austin, Eloise D; Yokoyama, Maho; Ouadi, Khadija; Feil, Edward; Thorpe, Harry A; Williams, Barnabas; Perkins, Mark; Peacock, Sharon J; Clarke, Stephen R; Dordel, Janina; Holden, Matthew; Votintseva, Antonina A; Bowden, Rory; Crook, Derrick W; Young, Bernadette C; Wilson, Daniel J; Recker, Mario; Massey, Ruth C (2015-09-02) - Journal article
    Bacterial virulence is a multifaceted trait where the interactions between pathogen and host factors affect the severity and outcome of the infection. Toxin secretion is central to the biology of many bacterial pathogens ...
  • Genome sequencing defines phylogeny and spread of methicillin-resistant Staphylococcus aureus in a high transmission setting 

    Tong, Steven Y C; Holden, Matthew T G; Nickerson, Emma K; Cooper, Ben S; Köser, Claudio U; Cori, Anne; Jombart, Thibaut; Cauchemez, Simon; Fraser, Christophe; Wuthiekanun, Vanaporn; Thaipadungpanit, Janjira; Hongsuwan, Maliwan; Day, Nicholas P; Limmathurotsakul, Direk; Parkhill, Julian; Peacock, Sharon J (2014-12-09) - Journal article
    Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infection. Whole-genome sequencing of MRSA has been used to define phylogeny and transmission in well-resourced healthcare settings, yet the ...
  • A novel hybrid SCCmec-mecC region in Staphylococcus sciuri 

    Harrison, Ewan M; Paterson, Gavin K; Holden, Matthew T G; Ba, Xiaoliang; Rolo, Joana; Morgan, Fiona J E; Pichon, Bruno; Kearns, Angela; Zadoks, Ruth N; Peacock, Sharon J; Parkhill, Julian; Holmes, Mark A (2014-04) - Journal article
    Methicillin resistance in Staphylococcus spp. results from the expression of an alternative penicillin-binding protein 2a (encoded by mecA) with a low affinity for β-lactam antibiotics. Recently, a novel variant of mecA ...
  • Population genetic structuring of methicillin-resistant Staphylococcus aureus clone EMRSA-15 within UK reflects patient referral patterns 

    Donker, Tjibbe; Reuter, Sandra; Scriberras, James; Reynolds, Rosy; Brown, Nicholas M; Török, M Estée; James, Richard; Network, East Of England Microbiology Research; Aanensen, David M; Bentley, Stephen D; Holden, Matthew T. G.; Parkhill, Julian; Spratt, Brian G; Peacock, Sharon J; Feil, Edward J; Grundmann, Hajo (2017-07-09) - Journal article
    Antibiotic resistance forms a serious threat to the health of hospitalised patients, rendering otherwise treatable bacterial infections potentially life-threatening. A thorough understanding of the mechanisms by which ...
  • Predicting the virulence of MRSA from its genome sequence 

    Laabei, Maisem; Recker, Mario; Rudkin, Justine K; Aldeljawi, Mona; Gulay, Zeynep; Sloan, Tim J; Williams, Paul; Endres, Jennifer L; Bayles, Kenneth W; Fey, Paul D; Yajjala, Vijaya Kumar; Widhelm, Todd; Hawkins, Erica; Lewis, Katie; Parfett, Sara; Scowen, Lucy; Peacock, Sharon J; Holden, Matthew; Wilson, Daniel; Read, Timothy D; van den Elsen, Jean; Priest, Nicholas K; Feil, Edward J; Hurst, Laurence D; Josefsson, Elisabet; Massey, Ruth C (2014-04-09) - Journal article
    Microbial virulence is a complex and often multifactorial phenotype, intricately linked to a pathogen's evolutionary trajectory. Toxicity, the ability to destroy host cell membranes, and adhesion, the ability to adhere to ...
  • A shared population of epidemic methicillin-resistant Staphylococcus aureus 15 circulates in humans and companion animals 

    Harrison, Ewan M; Weinert, Lucy A; Holden, Matthew T G; Welch, John J; Wilson, Katherine; Morgan, Fiona J E; Harris, Simon R; Loeffler, Anette; Boag, Amanda K; Peacock, Sharon J; Paterson, Gavin K; Waller, Andrew S; Parkhill, Julian; Holmes, Mark A (2014-05-13) - Journal article
    Methicillin-resistant Staphylococcus aureus (MRSA) is a global human health problem causing infections in both hospitals and the community. Companion animals, such as cats, dogs, and horses, are also frequently colonized ...
  • Whole-genome sequencing confirms that Burkholderia pseudomallei multilocus sequence types common to both Cambodia and Australia are due to homoplasy 

    De Smet, Birgit; Sarovich, Derek S; Price, Erin P; Mayo, Mark; Theobald, Vanessa; Kham, Chun; Heng, Seiha; Phe, Thong; Holden, Matthew T G; Parkhill, Julian; Peacock, Sharon J; Spratt, Brian G; Jacobs, Jan; Vandamme, Peter; Currie, Bart J (2015-01) - Journal article
    Burkholderia pseudomallei isolates with shared multilocus sequence types (STs) have not been isolated from different continents. We identified two STs shared between Australia and Cambodia. Whole-genome analysis revealed ...
  • Whole-genome sequencing to investigate a non-clonal melioidosis cluster on a remote Australian island 

    Sarovich, Derek S; Chapple, Stephanie N J; Price, Erin P; Mayo, Mark; Holden, Matthew T. G.; Peacock, Sharon J; Currie, Bart J (2017-08-31) - Journal article
    Melioidosis is a tropical disease caused by the bacterium Burkholderia pseudomallei. Outbreaks are uncommon and can generally be attributed to a single point source and strain. We used whole-genome sequencing to analyse ...