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dc.contributor.authorSarovich, Derek S
dc.contributor.authorChapple, Stephanie N J
dc.contributor.authorPrice, Erin P
dc.contributor.authorMayo, Mark
dc.contributor.authorHolden, Matthew T. G.
dc.contributor.authorPeacock, Sharon J
dc.contributor.authorCurrie, Bart J
dc.date.accessioned2017-11-13T17:30:08Z
dc.date.available2017-11-13T17:30:08Z
dc.date.issued2017-08-31
dc.identifier.citationSarovich , D S , Chapple , S N J , Price , E P , Mayo , M , Holden , M T G , Peacock , S J & Currie , B J 2017 , ' Whole-genome sequencing to investigate a non-clonal melioidosis cluster on a remote Australian island ' Microbial Genomics , vol. 3 , no. 8 , e000117 . https://doi.org/10.1099/mgen.0.000117en
dc.identifier.issn2057-5858
dc.identifier.otherPURE: 251486201
dc.identifier.otherPURE UUID: 9c44400a-2a62-4134-8294-6365f8595d3a
dc.identifier.otherPubMed: 29026657
dc.identifier.otherPubMedCentral: PMC5610713
dc.identifier.otherScopus: 85048564512
dc.identifier.otherORCID: /0000-0002-4958-2166/work/60196506
dc.identifier.otherWOS: 000431155400002
dc.identifier.urihttp://hdl.handle.net/10023/12075
dc.descriptionThis study was funded by National Health and Medical Research Council (Australia) grants 1046812 and 1098337. E. P. P. is in receipt of a University of the Sunshine Coast Research Fellowship.en
dc.description.abstractMelioidosis is a tropical disease caused by the bacterium Burkholderia pseudomallei. Outbreaks are uncommon and can generally be attributed to a single point source and strain. We used whole-genome sequencing to analyse B. pseudomallei isolates collected from an historical 2-year long case cluster that occurred in a remote northern Australian indigenous island community, where infections were previously linked to a contaminated communal water supply. We analysed the genome-wide relatedness of the two most common multilocus sequence types (STs) involved in the outbreak, STs 125 and 126. This analysis showed that although these STs were closely related on a whole-genome level, they demonstrated evidence of multiple recombination events that were unlikely to have occurred over the timeframe of the outbreak. Based on epidemiological and genetic data, we also identified two additional patients not previously associated with this outbreak. Our results confirm the previous hypothesis that a single unchlorinated water source harbouring multiple B. pseudomallei strains was linked to the outbreak, and that increased melioidosis risk in this community was associated with Piper methysticum root (kava) consumption.
dc.format.extent7
dc.language.isoeng
dc.relation.ispartofMicrobial Genomicsen
dc.rights© 2017 The Authors. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.en
dc.subjectMelioidosisen
dc.subjectSource tracingen
dc.subjectBurkholderia pseudomalleien
dc.subjectRecombinationen
dc.subjectPopulation geneticsen
dc.subjectOutbreaken
dc.subjectRA0421 Public health. Hygiene. Preventive Medicineen
dc.subjectDASen
dc.subject.lccRA0421en
dc.titleWhole-genome sequencing to investigate a non-clonal melioidosis cluster on a remote Australian islanden
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Infection Groupen
dc.contributor.institutionUniversity of St Andrews.Infection and Global Health Divisionen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1099/mgen.0.000117
dc.description.statusPeer revieweden


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