Biosynthesis of magnetic nanoparticles by human mesenchymal stem cells following transfection with the magnetotactic bacterial gene mms6
MetadataShow full item record
The use of stem cells to support tissue repair is facilitated by loading of the therapeutic cells with magnetic nanoparticles (MNPs) enabling magnetic tracking and targeting. Current methods for magnetizing cells use artificial MNPs and have disadvantages of variable uptake, cellular cytotoxicity and loss of nanoparticles on cell division. Here we demonstrate a transgenic approach to magnetize human mesenchymal stem cells (MSCs). MSCs are genetically modified by transfection with the mms6 gene derived from Magnetospirillum magneticum AMB-1, a magnetotactic bacterium that synthesises single-magnetic domain crystals which are incorporated into magnetosomes. Following transfection of MSCs with the mms6 gene there is bioassimilated synthesis of intracytoplasmic magnetic nanoparticles which can be imaged by MR and which have no deleterious effects on cell proliferation, migration or differentiation. The assimilation of magnetic nanoparticle synthesis into mammalian cells creates a real and compelling, cytocompatible, alternative to exogenous administration of MNPs.
Elfick , A , Rischitor , G , Mouras , R , Azfer , A , Lungaro , L , Uhlarz , M , Herrmannsdörfer , T , Lucocq , J M , Gamal , W , Bagnaninchi , P , Semple , S & Salter , D M 2017 , ' Biosynthesis of magnetic nanoparticles by human mesenchymal stem cells following transfection with the magnetotactic bacterial gene mms6 ' Scientific Reports , vol. 7 , 39755 . DOI: 10.1038/srep39755
Copyright the Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
DescriptionThis work was funded under the FP7 EU project “MAGISTER”. Grant Number: NMP3-LA-2008-214685 and additionally supported through a MRC Confidence in Concept scheme award to the University of Edinburgh.
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.