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dc.contributor.authorElfick, Alistair
dc.contributor.authorRischitor, Grigore
dc.contributor.authorMouras, Rabah
dc.contributor.authorAzfer, Asim
dc.contributor.authorLungaro, Lisa
dc.contributor.authorUhlarz, Marc
dc.contributor.authorHerrmannsdörfer, Thomas
dc.contributor.authorLucocq, John Milton
dc.contributor.authorGamal, Wesam
dc.contributor.authorBagnaninchi, Pierre
dc.contributor.authorSemple, Scott
dc.contributor.authorSalter, Donald M.
dc.identifier.citationElfick , A , Rischitor , G , Mouras , R , Azfer , A , Lungaro , L , Uhlarz , M , Herrmannsdörfer , T , Lucocq , J M , Gamal , W , Bagnaninchi , P , Semple , S & Salter , D M 2017 , ' Biosynthesis of magnetic nanoparticles by human mesenchymal stem cells following transfection with the magnetotactic bacterial gene mms6 ' , Scientific Reports , vol. 7 , 39755 .
dc.identifier.otherPURE: 248065201
dc.identifier.otherPURE UUID: 377c65d8-168e-4c26-baf8-5427b5822c3e
dc.identifier.otherScopus: 85008384451
dc.identifier.otherWOS: 000391129200001
dc.identifier.otherORCID: /0000-0002-5191-0093/work/64361254
dc.descriptionThis work was funded under the FP7 EU project “MAGISTER”. Grant Number: NMP3-LA-2008-214685 and additionally supported through a MRC Confidence in Concept scheme award to the University of Edinburgh.en
dc.description.abstractThe use of stem cells to support tissue repair is facilitated by loading of the therapeutic cells with magnetic nanoparticles (MNPs) enabling magnetic tracking and targeting. Current methods for magnetizing cells use artificial MNPs and have disadvantages of variable uptake, cellular cytotoxicity and loss of nanoparticles on cell division. Here we demonstrate a transgenic approach to magnetize human mesenchymal stem cells (MSCs). MSCs are genetically modified by transfection with the mms6 gene derived from Magnetospirillum magneticum AMB-1, a magnetotactic bacterium that synthesises single-magnetic domain crystals which are incorporated into magnetosomes. Following transfection of MSCs with the mms6 gene there is bioassimilated synthesis of intracytoplasmic magnetic nanoparticles which can be imaged by MR and which have no deleterious effects on cell proliferation, migration or differentiation. The assimilation of magnetic nanoparticle synthesis into mammalian cells creates a real and compelling, cytocompatible, alternative to exogenous administration of MNPs.
dc.relation.ispartofScientific Reportsen
dc.rightsCopyright the Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
dc.subjectQH301 Biologyen
dc.subjectRC0254 Neoplasms. Tumors. Oncology (including Cancer)en
dc.subjectRM Therapeutics. Pharmacologyen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.titleBiosynthesis of magnetic nanoparticles by human mesenchymal stem cells following transfection with the magnetotactic bacterial gene mms6en
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Cellular Medicine Divisionen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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