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dc.contributor.authorGiroux, Xavier
dc.contributor.authorMacNeill, Stuart
dc.date.accessioned2016-09-04T23:33:10Z
dc.date.available2016-09-04T23:33:10Z
dc.date.issued2015
dc.identifier.citationGiroux , X & MacNeill , S 2015 , ' A novel archaeal DNA repair factor that acts with the UvrABC system to repair mitomycin C-induced DNA damage in a PCNA-dependent manner ' , Molecular Microbiology . https://doi.org/10.1111/mmi.13210en
dc.identifier.issn0950-382X
dc.identifier.otherPURE: 214045682
dc.identifier.otherPURE UUID: c4b5df8d-e195-405c-8446-cbcf1eedc2f3
dc.identifier.otherScopus: 84955179044
dc.identifier.otherORCID: /0000-0002-0555-0007/work/39107857
dc.identifier.urihttp://hdl.handle.net/10023/9424
dc.descriptionThis work was supported by the USAF Office of Scientific Research under award number FA9550-10-1-0421.en
dc.description.abstractThe sliding clamp PCNA plays a vital role in a number of DNA repair pathways in eukaryotes and archaea by acting as a stable platform onto which other essential protein factors assemble. Many of these proteins interact with PCNA via a short peptide sequence known as a PIP (PCNA interacting protein) motif. Here we describe the identification and functional analysis of a novel PCNA interacting protein NreA that is conserved in the archaea and which has a PIP motif at its C-terminus. Using the genetically tractable euryarchaeon Haloferax volcanii as a model system, we show that the NreA protein is not required for cell viability but that loss of NreA (or replacement of the wild-type protein with a truncated version lacking the C-terminal PIP motif) results in an increased sensitivity to the DNA damaging agent mitomycin C (MMC) that correlates with delayed repair of MMC-induced chromosomal DNA damage monitored by pulsed-field gel electrophoresis (PFGE). Genetic epistasis analysis in Hfx. volcanii suggests that NreA works together with the UvrABC proteins in repairing DNA damage resulting from exposure to MMC. The wide distribution of NreA family members implies an important role for the protein in DNA damage repair in all archaeal lineages.
dc.language.isoeng
dc.relation.ispartofMolecular Microbiologyen
dc.rights© 2015 John Wiley & Sons Ltd. This work is made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at: https://dx.doi.org/10.1111/mmi.13210. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-820227.html#terms)en
dc.subjectDNA repairen
dc.subjectSliding clampen
dc.subjectDouble-strand breaksen
dc.subjectPCNAen
dc.subjectArchaeaen
dc.subjectQR Microbiologyen
dc.subject.lccQRen
dc.titleA novel archaeal DNA repair factor that acts with the UvrABC system to repair mitomycin C-induced DNA damage in a PCNA-dependent manneren
dc.typeJournal articleen
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1111/mmi.13210
dc.description.statusPeer revieweden
dc.date.embargoedUntil2016-09-04


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