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dc.contributor.authorHanding, Katarzyna B.
dc.contributor.authorShabalin, Ivan G
dc.contributor.authorKassaar, Omar
dc.contributor.authorKhazaipoul, Siavash
dc.contributor.authorBlindauer, Claudia A
dc.contributor.authorStewart, Alan James
dc.contributor.authorChruszcz, Maksymilian
dc.contributor.authorMinor, Wladek
dc.identifier.citationHanding , K B , Shabalin , I G , Kassaar , O , Khazaipoul , S , Blindauer , C A , Stewart , A J , Chruszcz , M & Minor , W 2016 , ' Circulatory zinc transport is controlled by distinct interdomain sites on mammalian albumins ' , Chemical Science , vol. 7 , no. 11 , pp. 6635-6648 .
dc.identifier.otherPURE: 245005018
dc.identifier.otherPURE UUID: ec093125-174c-482d-b9f3-e3416110577a
dc.identifier.otherScopus: 84992215930
dc.identifier.otherORCID: /0000-0003-4580-1840/work/60195795
dc.identifier.otherWOS: 000386228900005
dc.descriptionThe work described here was supported by NIH grants 1R01GM117325-01, 5U54GM094662-05 and R01GM053163, BBSRC grant BB/J006467/1 and British Heart Foundation grant PG/15/9/31270.en
dc.description.abstractZinc is an essential nutrient in the body; it is required for the catalytic activity of many hundreds of human enzymes and virtually all biological processes, therefore its homeostasis and trafficking is of crucial interest. Serum albumin is the major carrier of Zn2+ in the blood and is required for its systemic distribution. Here we present the first crystal structures of human serum albumin (HSA) and equine serum albumin (ESA) in complex with Zn2+. The structures allow unambiguous identification of the major zinc binding site on these two albumins, as well as several further, weaker zinc binding sites. The major site in both HSA and ESA has tetrahedral geometry and comprises three protein ligands from the sidechains of His67, His247 and Asp249 and a water molecule. Isothermal titration calorimetric studies of a HSA H67A mutant confirm this to be the highest affinity Zn2+ site. Furthermore, analysis of Zn2+ binding to HSA and ESA proved the presence of secondary sites with 20-50-fold weaker affinities, which may become of importance under particular physiological conditions. Both calorimetry and crystallography suggest that ESA possesses an additional site compared to HSA, involving Glu153, His157 and His288. The His157 residue is replaced by Phe in HSA, incapable of metal coordination. Collectively, these findings are critical to our understanding of the role serum albumin plays in circulatory Zn2+ handling and cellular delivery.
dc.relation.ispartofChemical Scienceen
dc.rightsThis journal is © The Royal Society of Chemistry 2016. Open Access Article. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.en
dc.subjectRC Internal medicineen
dc.subjectQD Chemistryen
dc.subjectQH301 Biologyen
dc.titleCirculatory zinc transport is controlled by distinct interdomain sites on mammalian albuminsen
dc.typeJournal articleen
dc.contributor.sponsorBritish Heart Foundationen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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