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dc.contributor.authorMorton, Andrew
dc.contributor.authorMurawski, Caroline
dc.contributor.authorPulver, Stefan
dc.contributor.authorGather, Malte Christian
dc.date.accessioned2016-08-04T09:30:11Z
dc.date.available2016-08-04T09:30:11Z
dc.date.issued2016-08-03
dc.identifier.citationMorton , A , Murawski , C , Pulver , S & Gather , M C 2016 , ' High-brightness organic light-emitting diodes for optogenetic control of Drosophila locomotor behaviour ' , Scientific Reports , vol. 6 , 31117 . https://doi.org/10.1038/srep31117en
dc.identifier.issn2045-2322
dc.identifier.otherPURE: 244482552
dc.identifier.otherPURE UUID: ac56ffa0-f48c-4d01-aaa7-abb8a68975ba
dc.identifier.otherScopus: 84981745035
dc.identifier.otherORCID: /0000-0002-4857-5562/work/47136466
dc.identifier.otherWOS: 000380652000001
dc.identifier.otherORCID: /0000-0001-5170-7522/work/69463430
dc.identifier.urihttp://hdl.handle.net/10023/9255
dc.descriptionWe thank Simone Lenk and Tobias Günther (TU Dresden) for fruitful discussions and technical support with OLED preparation. We are grateful for financial support from the Scottish Funding Council (through SUPA), Human Frontier Science Program (RGY0074/2013), Wellcome Trust Institutional Strategic Support Fund St Andrews and the RS Macdonald Charitable Trust. C.M. acknowledges funding by the European Commission through a Marie Sklodowska-Curie Individual Fellowship (703387).en
dc.description.abstractOrganic light emitting diodes (OLEDs) are in widespread use in today’s mobile phones and are likely to drive the next generation of large area displays and solid-state lighting. Here we show steps towards their utility as a platform technology for biophotonics, by demonstrating devices capable of optical controlling behaviour in live animals. Using devices with pin OLED architecture, sufficient illumination intensity (0.3 mW.mm-2) to activate channelrhodopsins (ChRs) in vivo was reliably achieved at low operating voltages (5 V). In Drosophila melanogaster third instar larvae expressing ChR2(H134R) in motor neurons, we found that pulsed illumination from blue and green OLEDs triggered robust and reversible contractions in animals. This response was temporally coupled to the timing of OLED illumination. With blue OLED illumination, the initial rate and overall size of the behavioural response was strongest. Green OLEDs achieved roughly 70% of the response observed with blue OLEDs. Orange OLEDs did not produce contractions in larvae, in agreement with the spectral response of ChR2(H134R). The device configuration presented here could be modified to accommodate other small model organisms, cell cultures or tissue slices and the ability of OLEDs to provide patterned illumination and spectral tuning can broaden their utility in optogenetics experiments further.
dc.format.extent8
dc.language.isoeng
dc.relation.ispartofScientific Reportsen
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.subjectQH301 Biologyen
dc.subjectQC Physicsen
dc.subjectDASen
dc.subject.lccQH301en
dc.subject.lccQCen
dc.titleHigh-brightness organic light-emitting diodes for optogenetic control of Drosophila locomotor behaviouren
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews.School of Psychology and Neuroscienceen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1038/srep31117
dc.description.statusPeer revieweden


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