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High-brightness organic light-emitting diodes for optogenetic control of Drosophila locomotor behaviour

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Morton_2016_High_brightness_srep31117.pdf (962.4Kb)
Date
03/08/2016
Author
Morton, Andrew
Murawski, Caroline
Pulver, Stefan
Gather, Malte Christian
Keywords
QH301 Biology
QC Physics
DAS
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Abstract
Organic light emitting diodes (OLEDs) are in widespread use in today’s mobile phones and are likely to drive the next generation of large area displays and solid-state lighting. Here we show steps towards their utility as a platform technology for biophotonics, by demonstrating devices capable of optical controlling behaviour in live animals. Using devices with pin OLED architecture, sufficient illumination intensity (0.3 mW.mm-2) to activate channelrhodopsins (ChRs) in vivo was reliably achieved at low operating voltages (5 V). In Drosophila melanogaster third instar larvae expressing ChR2(H134R) in motor neurons, we found that pulsed illumination from blue and green OLEDs triggered robust and reversible contractions in animals. This response was temporally coupled to the timing of OLED illumination. With blue OLED illumination, the initial rate and overall size of the behavioural response was strongest. Green OLEDs achieved roughly 70% of the response observed with blue OLEDs. Orange OLEDs did not produce contractions in larvae, in agreement with the spectral response of ChR2(H134R). The device configuration presented here could be modified to accommodate other small model organisms, cell cultures or tissue slices and the ability of OLEDs to provide patterned illumination and spectral tuning can broaden their utility in optogenetics experiments further.
Citation
Morton , A , Murawski , C , Pulver , S & Gather , M C 2016 , ' High-brightness organic light-emitting diodes for optogenetic control of Drosophila locomotor behaviour ' , Scientific Reports , vol. 6 , 31117 . https://doi.org/10.1038/srep31117
Publication
Scientific Reports
Status
Peer reviewed
DOI
https://doi.org/10.1038/srep31117
ISSN
2045-2322
Type
Journal article
Rights
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Description
We thank Simone Lenk and Tobias Günther (TU Dresden) for fruitful discussions and technical support with OLED preparation. We are grateful for financial support from the Scottish Funding Council (through SUPA), Human Frontier Science Program (RGY0074/2013), Wellcome Trust Institutional Strategic Support Fund St Andrews and the RS Macdonald Charitable Trust. C.M. acknowledges funding by the European Commission through a Marie Sklodowska-Curie Individual Fellowship (703387).
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  • University of St Andrews Research
URI
http://hdl.handle.net/10023/9255

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