Lasing within live cells containing intracellular optical micro-resonators for barcode-type cell tagging and tracking
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We report on a laser that is fully embedded into a single live cell. By harnessing natural endocytosis of the cell we introduce a fluorescent whispering gallery mode (WGM) micro-resonator into the cell cytoplasm. On pumping with nanojoule light pulses, green laser emission is generated inside the cells. Our approach can be applied to different cell types and cells with micro-resonators remain viable for weeks under standard conditions. The characteristics of the lasing spectrum provide each cell with a barcode-type label which enables uniquely identifying and tracking of individual migrating cells. Self-sustained lasing from cells paves the way to new forms of cell tracking, intracellular sensing and adaptive imaging.
Schubert , M , Steude , A , Liehm , P , Kronenberg , N M , Karl , M , Campbell , E C , Powis , S J & Gather , M C 2015 , ' Lasing within live cells containing intracellular optical micro-resonators for barcode-type cell tagging and tracking ' Nano Letters , vol 15 , no. 8 , pp. 5674-5652 . DOI: 10.1021/acs.nanolett.5b02491
Copyright 2015 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Nano Letters, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/acs.nanolett.5b02491
This work was supported by the European Union Marie Curie Career Integration Grant (PCIG12-GA-2012-334407) and the Scottish Funding Council (SUPA II). M.S. acknowledges funding by the German Science Foundation (DFG) through a Research Fellowship (SCHU 3003/1-1).
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