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dc.contributor.authorHammond, Robert J H
dc.contributor.authorBaron, Vincent O
dc.contributor.authorOravcova, Katarina
dc.contributor.authorLipworth, Sam
dc.contributor.authorGillespie, Stephen H
dc.date.accessioned2016-07-08T23:32:11Z
dc.date.available2016-07-08T23:32:11Z
dc.date.issued2015-07-09
dc.identifier.citationHammond , R J H , Baron , V O , Oravcova , K , Lipworth , S & Gillespie , S H 2015 , ' Phenotypic resistance in mycobacteria : is it because I am old or fat that I resist you? ' , Journal of Antimicrobial Chemotherapy , vol. Advance Access . https://doi.org/10.1093/jac/dkv178en
dc.identifier.issn0305-7453
dc.identifier.otherPURE: 194099904
dc.identifier.otherPURE UUID: 170e6359-8830-4dba-bb5c-8b72197a14a7
dc.identifier.otherPubMed: 26163401
dc.identifier.otherPubMed: 26163401
dc.identifier.otherScopus: 84943747320
dc.identifier.otherORCID: /0000-0001-6537-7712/work/39477883
dc.identifier.otherWOS: 000363249800020
dc.identifier.urihttp://hdl.handle.net/10023/9101
dc.description.abstractSynopsis Objectives We aimed to explore the phenomenon of phenotypic resistance to anti-mycobacterial antibiotics and to determine whether this was associated with cell age or presence of lipid bodies. Methods The accumulation of lipid body positive cells (lipid rich- LR) was followed using cell staining and flow cytometry. LR cells of M. smegmatis, M. marinum, M. fortuitum and BGC were separated from non-lipid body containing cells (lipid poor- LP) and their MBC determined. We also compared the MBC of LR and LP from “old” and “young” cultures. Results The LR cells of all species were more resistant to antibiotics than LP cells. For Mycobacterium bovis (BCG) the susceptibility ratios were as follows; Rifampicin-5X, isoniazid-16.7X, ethambutol-5X, ciprofloxacin-5X. Phenotypic resistance was found in LR cells irrespective of cell age. Conclusions We have shown that phenotypic antibiotic resistance is associated with the presence of lipid bodies irrespective of cell age. These data have important implications for our understanding of relapse in mycobacterial infections.
dc.language.isoeng
dc.relation.ispartofJournal of Antimicrobial Chemotherapyen
dc.rightsThis is a pre-copyedited, author-produced PDF of an article accepted for publication in Journal of Antimicrobial Chemotherapy following peer review. The version of record Phenotypic resistance in mycobacteria: is it because I am old or fat that I resist you? Hammond, R. J. H., Baron, V. O., Oravcova, K., Lipworth, S. & Gillespie, S. H. 9 Jul 2015 In : Journal of Antimicrobial Chemotherapy is available online at: http://jac.oxfordjournals.org/content/early/2015/07/09/jac.dkv178en
dc.subjectMycobacterium tuberculosisen
dc.subjectAntibiotic resistanceen
dc.subjectLipid richen
dc.subjectLipid pooren
dc.subjectR Medicineen
dc.subjectNDASen
dc.subject.lccRen
dc.titlePhenotypic resistance in mycobacteria : is it because I am old or fat that I resist you?en
dc.typeJournal articleen
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Global Health Implementation Groupen
dc.contributor.institutionUniversity of St Andrews.Gillespie Groupen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.Infection Groupen
dc.identifier.doihttps://doi.org/10.1093/jac/dkv178
dc.description.statusPeer revieweden
dc.date.embargoedUntil2016-07-09


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