St Andrews Research Repository

St Andrews University Home
View Item 
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  • Login
JavaScript is disabled for your browser. Some features of this site may not work without it.

Phenotypic resistance in mycobacteria : is it because I am old or fat that I resist you?

Thumbnail
View/Open
Is_it_Because_I_am_Old_or_Fat_that_you_Resist_me_V7.4_final_WITH_FIGURES_no_biopsy_SHG2.pdf (537.4Kb)
Date
09/07/2015
Author
Hammond, Robert J H
Baron, Vincent O
Oravcova, Katarina
Lipworth, Sam
Gillespie, Stephen H
Keywords
Mycobacterium tuberculosis
Antibiotic resistance
Lipid rich
Lipid poor
R Medicine
NDAS
Metadata
Show full item record
Altmetrics Handle Statistics
Altmetrics DOI Statistics
Abstract
Synopsis Objectives We aimed to explore the phenomenon of phenotypic resistance to anti-mycobacterial antibiotics and to determine whether this was associated with cell age or presence of lipid bodies. Methods The accumulation of lipid body positive cells (lipid rich- LR) was followed using cell staining and flow cytometry. LR cells of M. smegmatis, M. marinum, M. fortuitum and BGC were separated from non-lipid body containing cells (lipid poor- LP) and their MBC determined. We also compared the MBC of LR and LP from “old” and “young” cultures. Results The LR cells of all species were more resistant to antibiotics than LP cells. For Mycobacterium bovis (BCG) the susceptibility ratios were as follows; Rifampicin-5X, isoniazid-16.7X, ethambutol-5X, ciprofloxacin-5X. Phenotypic resistance was found in LR cells irrespective of cell age. Conclusions We have shown that phenotypic antibiotic resistance is associated with the presence of lipid bodies irrespective of cell age. These data have important implications for our understanding of relapse in mycobacterial infections.
Citation
Hammond , R J H , Baron , V O , Oravcova , K , Lipworth , S & Gillespie , S H 2015 , ' Phenotypic resistance in mycobacteria : is it because I am old or fat that I resist you? ' , Journal of Antimicrobial Chemotherapy , vol. Advance Access . https://doi.org/10.1093/jac/dkv178
Publication
Journal of Antimicrobial Chemotherapy
Status
Peer reviewed
DOI
https://doi.org/10.1093/jac/dkv178
ISSN
0305-7453
Type
Journal article
Rights
This is a pre-copyedited, author-produced PDF of an article accepted for publication in Journal of Antimicrobial Chemotherapy following peer review. The version of record Phenotypic resistance in mycobacteria: is it because I am old or fat that I resist you? Hammond, R. J. H., Baron, V. O., Oravcova, K., Lipworth, S. & Gillespie, S. H. 9 Jul 2015 In : Journal of Antimicrobial Chemotherapy is available online at: http://jac.oxfordjournals.org/content/early/2015/07/09/jac.dkv178
Collections
  • University of St Andrews Research
URI
http://hdl.handle.net/10023/9101

Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

Advanced Search

Browse

All of RepositoryCommunities & CollectionsBy Issue DateNamesTitlesSubjectsClassificationTypeFunderThis CollectionBy Issue DateNamesTitlesSubjectsClassificationTypeFunder

My Account

Login

Open Access

To find out how you can benefit from open access to research, see our library web pages and Open Access blog. For open access help contact: openaccess@st-andrews.ac.uk.

Accessibility

Read our Accessibility statement.

How to submit research papers

The full text of research papers can be submitted to the repository via Pure, the University's research information system. For help see our guide: How to deposit in Pure.

Electronic thesis deposit

Help with deposit.

Repository help

For repository help contact: Digital-Repository@st-andrews.ac.uk.

Give Feedback

Cookie policy

This site may use cookies. Please see Terms and Conditions.

Usage statistics

COUNTER-compliant statistics on downloads from the repository are available from the IRUS-UK Service. Contact us for information.

© University of St Andrews Library

University of St Andrews is a charity registered in Scotland, No SC013532.

  • Facebook
  • Twitter