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dc.contributor.authorEspada, Margarida
dc.contributor.authorSilva, Ana Cláudia
dc.contributor.authorEves van den Akker, Sebastian
dc.contributor.authorCock, Peter J. A.
dc.contributor.authorMota, Manuel
dc.contributor.authorJones, John T.
dc.identifier.citationEspada , M , Silva , A C , Eves van den Akker , S , Cock , P J A , Mota , M & Jones , J T 2016 , ' Identification and characterization of parasitism genes from the pinewood nematode Bursaphelenchus xylophilus reveals a multilayered detoxification strategy ' , Molecular Plant Pathology , vol. 17 , no. 2 , pp. 286-295 .
dc.identifier.otherPURE: 241082620
dc.identifier.otherPURE UUID: 46fa6d93-85ff-4085-a870-14954be84cf1
dc.identifier.otherScopus: 84956575961
dc.identifier.otherWOS: 000368704300013
dc.descriptionThis work was supported by the REPHRAME project (KBBE.2010.1.4-09). ME is funded by FCT (Fundação para a Ciência e a Tecnologia, IP) under a PhD grant (SFRH/BD/84541/2012). ME and MM are also funded by FEDER Funds through the Operational Programme for Competitiveness Factors—COMPETE and National Funds through FCT—Foundation for Science and Technology under the Strategic Projects PEst-C/AGR/UI0115/2011 and PEst-OE/AGR/UI0115/2014. AS was funded by an ERASMUS MUNDUS Category B scholarship awarded through project 2008–2102 (EUMAINE). The James Hutton Institute receives funding from the Scottish Government Rural and Environmental Science and Analytical Services division (RESAS).en
dc.description.abstractThe migratory endoparasitic nematode Bursaphelenchus xylophilus, which is the causal agent of pine wilt disease, has phytophagous and mycetophagous phases during its life cycle. This highly unusual feature distinguishes it from other plant-parasitic nematodes and requires profound changes in biology between modes. During the phytophagous stage, the nematode migrates within pine trees, feeding on the contents of parenchymal cells. Like other plant pathogens, B.xylophilus secretes effectors from pharyngeal gland cells into the host during infection. We provide the first description of changes in the morphology of these gland cells between juvenile and adult life stages. Using a comparative transcriptomics approach and an effector identification pipeline, we identify numerous novel parasitism genes which may be important for the mediation of interactions of B.xylophilus with its host. In-depth characterization of all parasitism genes using in situ hybridization reveals two major categories of detoxification proteins, those specifically expressed in either the pharyngeal gland cells or the digestive system. These data suggest that B.xylophilus incorporates effectors in a multilayer detoxification strategy in order to protect itself from host defence responses during phytophagy.
dc.relation.ispartofMolecular Plant Pathologyen
dc.rights© 2015 BSPP AND JOHN WILEY & SONS LTD. This work is made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at
dc.subjectBursaphelenchus xylophilusen
dc.subjectGland cellsen
dc.subjectXenobiotic metabolismen
dc.subjectQH301 Biologyen
dc.subjectPlant Scienceen
dc.subjectAgronomy and Crop Scienceen
dc.subjectSoil Scienceen
dc.subjectMolecular Biologyen
dc.titleIdentification and characterization of parasitism genes from the pinewood nematode Bursaphelenchus xylophilus reveals a multilayered detoxification strategyen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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