Show simple item record

Files in this item

Thumbnail

Item metadata

dc.contributor.authorPaquet, Nicolas
dc.contributor.authorAdams, Mark N
dc.contributor.authorAshton, Nicholas W
dc.contributor.authorTouma, Christine
dc.contributor.authorGamsjaeger, Roland
dc.contributor.authorCubeddu, Liza
dc.contributor.authorLeong, Vincent
dc.contributor.authorBeard, Sam
dc.contributor.authorBolderson, Emma
dc.contributor.authorBotting, Catherine H
dc.contributor.authorO'Byrne, Kenneth J
dc.contributor.authorRichard, Derek J
dc.date.accessioned2016-06-15T11:30:04Z
dc.date.available2016-06-15T11:30:04Z
dc.date.issued2016-06-08
dc.identifier.citationPaquet , N , Adams , M N , Ashton , N W , Touma , C , Gamsjaeger , R , Cubeddu , L , Leong , V , Beard , S , Bolderson , E , Botting , C H , O'Byrne , K J & Richard , D J 2016 , ' hSSB1 (NABP2/OBFC2B) is regulated by oxidative stress ' , Scientific Reports , vol. 6 , 27466 . https://doi.org/10.1038/srep27446en
dc.identifier.issn2045-2322
dc.identifier.otherPURE: 243430143
dc.identifier.otherPURE UUID: f4a0c935-021c-46b8-84b4-a747e533f6d8
dc.identifier.otherPubMed: 27273218
dc.identifier.otherScopus: 84976383986
dc.identifier.otherWOS: 000377461900001
dc.identifier.urihttp://hdl.handle.net/10023/8987
dc.description.abstractThe maintenance of genome stability is an essential cellular process to prevent the development of diseases including cancer. hSSB1 (NABP2/ OBFC2A) is a critical component of the DNA damage response where it participates in the repair of double-strand DNA breaks and in base excision repair of oxidized guanine residues (8-oxoguanine) by aiding the localization of the human 8-oxoguanine glycosylase (hOGG1) to damaged DNA. Here we demonstrate that following oxidative stress, hSSB1 is stabilized as an oligomer which is required for hSSB1 to function in the removal of 8-oxoguanine. Monomeric hSSB1 shows a decreased affinity for oxidized DNA resulting in a cellular 8-oxoguanine-repair defect and in the absence of ATM signaling initiation. While hSSB1 oligomerization is important for the removal of 8-oxoguanine from the genome, it is not required for the repair of double-strand DNA-breaks by homologous recombination. These findings demonstrate a novel hSSB1 regulatory mechanism for the repair of damaged DNA.
dc.language.isoeng
dc.relation.ispartofScientific Reportsen
dc.rightsCopyright 2016 the Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.subjectQD Chemistryen
dc.subjectQH301 Biologyen
dc.subjectNDASen
dc.subject.lccQDen
dc.subject.lccQH301en
dc.titlehSSB1 (NABP2/OBFC2B) is regulated by oxidative stressen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.School of Chemistryen
dc.contributor.institutionUniversity of St Andrews.EaSTCHEMen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1038/srep27446
dc.description.statusPeer revieweden
dc.identifier.urlhttp://www.nature.com/articles/srep27446?WT.feed_name=subjects_biological-sciences#supplementary-informationen


This item appears in the following Collection(s)

Show simple item record