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hSSB1 (NABP2/OBFC2B) is regulated by oxidative stress

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Botting_2016_SciRep_hSSB1_CCBY_FinalPublishedVersion.pdf (1.568Mb)
Date
08/06/2016
Author
Paquet, Nicolas
Adams, Mark N
Ashton, Nicholas W
Touma, Christine
Gamsjaeger, Roland
Cubeddu, Liza
Leong, Vincent
Beard, Sam
Bolderson, Emma
Botting, Catherine H
O'Byrne, Kenneth J
Richard, Derek J
Keywords
QD Chemistry
QH301 Biology
NDAS
Metadata
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Abstract
The maintenance of genome stability is an essential cellular process to prevent the development of diseases including cancer. hSSB1 (NABP2/ OBFC2A) is a critical component of the DNA damage response where it participates in the repair of double-strand DNA breaks and in base excision repair of oxidized guanine residues (8-oxoguanine) by aiding the localization of the human 8-oxoguanine glycosylase (hOGG1) to damaged DNA. Here we demonstrate that following oxidative stress, hSSB1 is stabilized as an oligomer which is required for hSSB1 to function in the removal of 8-oxoguanine. Monomeric hSSB1 shows a decreased affinity for oxidized DNA resulting in a cellular 8-oxoguanine-repair defect and in the absence of ATM signaling initiation. While hSSB1 oligomerization is important for the removal of 8-oxoguanine from the genome, it is not required for the repair of double-strand DNA-breaks by homologous recombination. These findings demonstrate a novel hSSB1 regulatory mechanism for the repair of damaged DNA.
Citation
Paquet , N , Adams , M N , Ashton , N W , Touma , C , Gamsjaeger , R , Cubeddu , L , Leong , V , Beard , S , Bolderson , E , Botting , C H , O'Byrne , K J & Richard , D J 2016 , ' hSSB1 (NABP2/OBFC2B) is regulated by oxidative stress ' , Scientific Reports , vol. 6 , 27466 . https://doi.org/10.1038/srep27446
Publication
Scientific Reports
Status
Peer reviewed
DOI
https://doi.org/10.1038/srep27446
ISSN
2045-2322
Type
Journal article
Rights
Copyright 2016 the Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Collections
  • University of St Andrews Research
URL
http://www.nature.com/articles/srep27446?WT.feed_name=subjects_biological-sciences#supplementary-information
URI
http://hdl.handle.net/10023/8987

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