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A compact light-sheet microscope for the study of the mammalian central nervous system
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dc.contributor.author | Yang, Zhengyi | |
dc.contributor.author | Haslehurst, Peter | |
dc.contributor.author | Scott, Suzanne | |
dc.contributor.author | Emptage, Nigel | |
dc.contributor.author | Dholakia, Kishan | |
dc.date.accessioned | 2016-06-01T15:30:06Z | |
dc.date.available | 2016-06-01T15:30:06Z | |
dc.date.issued | 2016-05-24 | |
dc.identifier | 242102980 | |
dc.identifier | 2f781e80-2de4-4d29-9ede-7b3dded1ac31 | |
dc.identifier | 84971351690 | |
dc.identifier | 000376362500001 | |
dc.identifier.citation | Yang , Z , Haslehurst , P , Scott , S , Emptage , N & Dholakia , K 2016 , ' A compact light-sheet microscope for the study of the mammalian central nervous system ' , Scientific Reports , vol. 6 , 26317 . https://doi.org/10.1038/srep26317 | en |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://hdl.handle.net/10023/8911 | |
dc.description | We thank the UK Engineering and Physical Sciences Research Council (EPSRC) (grant number EP/J01771X/1), Biotechnology and Biological Sciences Research Council (BBSRC) and the Wellcome Trust for funding. | en |
dc.description.abstract | Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45◦ inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community. | |
dc.format.extent | 7 | |
dc.format.extent | 891634 | |
dc.language.iso | eng | |
dc.relation.ispartof | Scientific Reports | en |
dc.subject | RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry | en |
dc.subject | QC Physics | en |
dc.subject | DAS | en |
dc.subject.lcc | RC0321 | en |
dc.subject.lcc | QC | en |
dc.title | A compact light-sheet microscope for the study of the mammalian central nervous system | en |
dc.type | Journal article | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | https://doi.org/10.1038/srep26317 | |
dc.description.status | Peer reviewed | en |
dc.identifier.url | http://www.nature.com/articles/srep26317 | en |
dc.identifier.grantnumber | EP/J01771X/1 | en |
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