Files in this item
A compact light-sheet microscope for the study of the mammalian central nervous system
Item metadata
dc.contributor.author | Yang, Zhengyi | |
dc.contributor.author | Haslehurst, Peter | |
dc.contributor.author | Scott, Suzanne | |
dc.contributor.author | Emptage, Nigel | |
dc.contributor.author | Dholakia, Kishan | |
dc.date.accessioned | 2016-06-01T15:30:06Z | |
dc.date.available | 2016-06-01T15:30:06Z | |
dc.date.issued | 2016-05-24 | |
dc.identifier.citation | Yang , Z , Haslehurst , P , Scott , S , Emptage , N & Dholakia , K 2016 , ' A compact light-sheet microscope for the study of the mammalian central nervous system ' , Scientific Reports , vol. 6 , 26317 . https://doi.org/10.1038/srep26317 | en |
dc.identifier.issn | 2045-2322 | |
dc.identifier.other | PURE: 242102980 | |
dc.identifier.other | PURE UUID: 2f781e80-2de4-4d29-9ede-7b3dded1ac31 | |
dc.identifier.other | Scopus: 84971351690 | |
dc.identifier.other | WOS: 000376362500001 | |
dc.identifier.uri | https://hdl.handle.net/10023/8911 | |
dc.description | We thank the UK Engineering and Physical Sciences Research Council (EPSRC) (grant number EP/J01771X/1), Biotechnology and Biological Sciences Research Council (BBSRC) and the Wellcome Trust for funding. | en |
dc.description.abstract | Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45◦ inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community. | |
dc.format.extent | 7 | |
dc.language.iso | eng | |
dc.relation.ispartof | Scientific Reports | en |
dc.rights | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | en |
dc.subject | RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry | en |
dc.subject | QC Physics | en |
dc.subject | DAS | en |
dc.subject.lcc | RC0321 | en |
dc.subject.lcc | QC | en |
dc.title | A compact light-sheet microscope for the study of the mammalian central nervous system | en |
dc.type | Journal article | en |
dc.contributor.sponsor | EPSRC | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | https://doi.org/10.1038/srep26317 | |
dc.description.status | Peer reviewed | en |
dc.identifier.url | http://www.nature.com/articles/srep26317 | en |
dc.identifier.grantnumber | EP/J01771X/1 | en |
This item appears in the following Collection(s)
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.