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dc.contributor.authorYang, Zhengyi
dc.contributor.authorHaslehurst, Peter
dc.contributor.authorScott, Suzanne
dc.contributor.authorEmptage, Nigel
dc.contributor.authorDholakia, Kishan
dc.date.accessioned2016-06-01T15:30:06Z
dc.date.available2016-06-01T15:30:06Z
dc.date.issued2016-05-24
dc.identifier.citationYang , Z , Haslehurst , P , Scott , S , Emptage , N & Dholakia , K 2016 , ' A compact light-sheet microscope for the study of the mammalian central nervous system ' , Scientific Reports , vol. 6 , 26317 . https://doi.org/10.1038/srep26317en
dc.identifier.issn2045-2322
dc.identifier.otherPURE: 242102980
dc.identifier.otherPURE UUID: 2f781e80-2de4-4d29-9ede-7b3dded1ac31
dc.identifier.otherScopus: 84971351690
dc.identifier.otherWOS: 000376362500001
dc.identifier.urihttp://hdl.handle.net/10023/8911
dc.descriptionWe thank the UK Engineering and Physical Sciences Research Council (EPSRC) (grant number EP/J01771X/1), Biotechnology and Biological Sciences Research Council (BBSRC) and the Wellcome Trust for funding.en
dc.description.abstractInvestigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45◦ inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community.
dc.format.extent7
dc.language.isoeng
dc.relation.ispartofScientific Reportsen
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.subjectRC0321 Neuroscience. Biological psychiatry. Neuropsychiatryen
dc.subjectQC Physicsen
dc.subjectDASen
dc.subject.lccRC0321en
dc.subject.lccQCen
dc.titleA compact light-sheet microscope for the study of the mammalian central nervous systemen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1038/srep26317
dc.description.statusPeer revieweden
dc.identifier.urlhttp://www.nature.com/articles/srep26317en


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