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A compact light-sheet microscope for the study of the mammalian central nervous system

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Yang_2016_ACompact_SREP_CC.pdf (870.7Kb)
Date
24/05/2016
Author
Yang, Zhengyi
Haslehurst, Peter
Scott, Suzanne
Emptage, Nigel
Dholakia, Kishan
Keywords
RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
QC Physics
DAS
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Abstract
Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45◦ inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community.
Citation
Yang , Z , Haslehurst , P , Scott , S , Emptage , N & Dholakia , K 2016 , ' A compact light-sheet microscope for the study of the mammalian central nervous system ' , Scientific Reports , vol. 6 , 26317 . https://doi.org/10.1038/srep26317
Publication
Scientific Reports
Status
Peer reviewed
DOI
https://doi.org/10.1038/srep26317
ISSN
2045-2322
Type
Journal article
Rights
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Description
We thank the UK Engineering and Physical Sciences Research Council (EPSRC) (grant number EP/J01771X/1), Biotechnology and Biological Sciences Research Council (BBSRC) and the Wellcome Trust for funding.
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  • University of St Andrews Research
URL
http://www.nature.com/articles/srep26317
URI
http://hdl.handle.net/10023/8911

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