A compact light-sheet microscope for the study of the mammalian central nervous system
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Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45◦ inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community.
Yang , Z , Haslehurst , P , Scott , S , Emptage , N & Dholakia , K 2016 , ' A compact light-sheet microscope for the study of the mammalian central nervous system ' Scientific Reports , vol 6 , 26317 . DOI: 10.1038/srep26317
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We thank the UK Engineering and Physical Sciences Research Council (EPSRC) (grant number EP/J01771X/1), Biotechnology and Biological Sciences Research Council (BBSRC) and the Wellcome Trust for funding.