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dc.contributor.authorSteude, Anja
dc.contributor.authorWitts, Emily Charlotte
dc.contributor.authorMiles, Gareth Brian
dc.contributor.authorGather, Malte Christian
dc.date.accessioned2016-05-11T08:30:09Z
dc.date.available2016-05-11T08:30:09Z
dc.date.issued2016-05-06
dc.identifier.citationSteude , A , Witts , E C , Miles , G B & Gather , M C 2016 , ' Arrays of microscopic organic LEDs for high resolution optogenetics ' Science Advances , vol. 2 , no. 5 , e1600061 . https://doi.org/10.1126/sciadv.1600061en
dc.identifier.issn2375-2548
dc.identifier.otherPURE: 242455971
dc.identifier.otherPURE UUID: 15a1ce99-548c-4d96-a244-556da69618d7
dc.identifier.otherScopus: 85006048790
dc.identifier.otherORCID: /0000-0002-8624-4625/work/29135002
dc.identifier.otherORCID: /0000-0002-4857-5562/work/47136423
dc.identifier.urihttp://hdl.handle.net/10023/8772
dc.descriptionWe thank A. Morton and C. Murawski (both University of St Andrews) and B.Richter (Fraunhofer FEP, Dresden) for fruitful discussions. HEK-293 cells that were stably transfected with ChR2-H134R-EYFP DNA were provided by M. Antkowiak and F. J. Gunn-Moore (both University of St Andrews). Funding: This work was supported by the Scottish Funding Council (via Scottish Universities Physics Alliance), the Human Frontier Science Program (RGY0074/2013), and the RS Macdonald Charitable Trust. Author contributions: A.S. performed the optogenetics experiments and data analysis. E.C.W. and G.B.M. carried out the patch clamp measurements. M.C.G. conceived and supervised the project. A.S. and M.C.G. jointly wrote the manuscript with input from all authors. Competing interests: The authors declare that they have no competing interests. Data and materials availability: All data needed to evaluate the conclusions in the paper are present in the paper. The research data supporting this publication can be accessed at DOI 10.17630/d758df2c-78ee-482c-ae7f-af37b00fdb52. Additional data related to this paper are available upon request from M.C.G. (mcg6@st-andrews.ac.uk).en
dc.description.abstractOptogenetics is a paradigm changing new method to study and manipulate the behavior of cells with light. Following major advances of the used genetic constructs over the last decade, the light sources required for optogenetic control are now receiving increased attention. Here, we report on a novel optogenetic illumination platform based on high density arrays of microscopic organic light emitting diodes (OLEDs). Due to the small dimensions of each array element (6x9 µm²) and the use of ultra-thin device encapsulation, these arrays enable illumination of cells with unprecedented spatiotemporal resolution. We show that adherent eukaryotic cells readily proliferate on these arrays and we demonstrate specific light-induced control of the ionic current across the membrane of individual live cells expressing different optogenetic constructs. Our work paves the way for the use of OLEDs for cell-specific optogenetic control in cultured neuronal networks, acute brain slices or as implants in vivo.
dc.format.extent8
dc.language.isoeng
dc.relation.ispartofScience Advancesen
dc.rightsCopyright © 2016, The Authors This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.en
dc.subjectOLEDen
dc.subjectOptogeneticsen
dc.subjectMicrodisplaysen
dc.subjectOrganic electronicsen
dc.subjectNeuroscienceen
dc.subjectChannelrhodopsinen
dc.subjectRC0321 Neuroscience. Biological psychiatry. Neuropsychiatryen
dc.subjectQC Physicsen
dc.subjectT Technologyen
dc.subjectDASen
dc.subject.lccRC0321en
dc.subject.lccQCen
dc.subject.lccTen
dc.titleArrays of microscopic organic LEDs for high resolution optogeneticsen
dc.typeJournal articleen
dc.description.versionPostprinten
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews.School of Psychology and Neuroscienceen
dc.contributor.institutionUniversity of St Andrews.Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1126/sciadv.1600061
dc.description.statusPeer revieweden


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