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dc.contributor.authorPalomares-Rius, Juan Emilio
dc.contributor.authorHedley, Pete
dc.contributor.authorCock, Peter J A
dc.contributor.authorMorris, Jenny A.
dc.contributor.authorJones, John T.
dc.contributor.authorBlok, Vivian C.
dc.date.accessioned2016-02-25T15:40:07Z
dc.date.available2016-02-25T15:40:07Z
dc.date.issued2016-02-04
dc.identifier.citationPalomares-Rius , J E , Hedley , P , Cock , P J A , Morris , J A , Jones , J T & Blok , V C 2016 , ' Gene expression changes in diapause or quiescent potato cyst nematode, Globodera pallida , eggs after hydration or exposure to tomato root diffusate ' PeerJ , no. 2 , 1654 . DOI: 10.7717/peerj.1654en
dc.identifier.issn2167-8359
dc.identifier.otherPURE: 241227341
dc.identifier.otherPURE UUID: 3232f678-7f32-4926-9633-e70ad1653cb5
dc.identifier.otherScopus: 84957555804
dc.identifier.urihttp://hdl.handle.net/10023/8314
dc.descriptionThe authors thank the Education Spanish Ministry for the grant provided for the first author under the "Ayudas para la movilidad postdoctoral en centros extranjeros'' scheme. The James Hutton Institute receives funding from the Scottish Government.en
dc.description.abstractPlant-parasitic nematodes (PPN) need to be adapted to survive in the absence of a suitable host or in hostile environmental conditions. Various forms of developmental arrest including hatching inhibition and dauer stages are used by PPN in order to survive these conditions and spread to other areas. Potato cyst nematodes (PCN) (Globodera pallida and G. rostochiensis) are frequently in an anhydrobiotic state, with unhatched nematode persisting for extended periods of time inside the cyst in the absence of the host. This paper shows fundamental changes in the response of quiescent and diapaused eggs of G. pallida to hydration and following exposure to tomato root diffusate (RD) using microarray gene expression analysis encompassing a broad set of genes. For the quiescent eggs, 547 genes showed differential expression following hydration vs. hydratation and RD (H-RD) treatment whereas 708 genes showed differential regulation for the diapaused eggs following these treatments. The comparison between hydrated quiescent and diapaused eggs showed marked differences, with 2,380 genes that were differentially regulated compared with 987 genes following H-RD. Hydrated quiescent and diapaused eggs were markedly different indicating differences in adaptation for long-term survival. Transport activity is highly up-regulated following H-RD and few genes were coincident between both kinds of eggs. With the quiescent eggs, the majority of genes were related to ion transport (mainly sodium), while the diapaused eggs showed a major diversity of transporters (amino acid transport, ion transport, acetylcholine or other molecules).en
dc.format.extent23en
dc.language.isoeng
dc.relation.ispartofPeerJen
dc.rights© 2016 Palomares-Rius et al. This is an open access article distributed under Creative Commons CC-BY 4.0 licence.en
dc.subjectMicroarrayen
dc.subjectGlobodera pallidaen
dc.subjectHatchingen
dc.subjectQuiescenceen
dc.subjectDiapauseen
dc.subjectGene expressionen
dc.subjectNematodesen
dc.subjectQH301 Biologyen
dc.subjectQH426 Geneticsen
dc.subjectS Agriculture (General)en
dc.subjectAgricultural and Biological Sciences(all)en
dc.subjectBiochemistry, Genetics and Molecular Biology(all)en
dc.subjectMedicine(all)en
dc.subjectNeuroscience(all)en
dc.subject.lccQH301en
dc.subject.lccQH426en
dc.subject.lccS1en
dc.titleGene expression changes in diapause or quiescent potato cyst nematode, Globodera pallida, eggs after hydration or exposure to tomato root diffusateen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.identifier.doihttp://dx.doi.org/10.7717/peerj.1654
dc.description.statusPeer revieweden


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