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Pumilio-based RNA in vivo imaging

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dc.contributor.authorTilsner, Jens
dc.date.accessioned2016-01-05T10:04:29Z
dc.date.available2016-01-05T10:04:29Z
dc.date.issued2015
dc.identifier157309879
dc.identifier3b15a3a6-5d82-4ad1-9812-fc72ed71fd5c
dc.identifier000343252500021
dc.identifier84921804074
dc.identifier.citationTilsner , J 2015 , ' Pumilio-based RNA in vivo imaging ' , Methods in Molecular Biology , vol. 1217 , pp. 295-328 . https://doi.org/10.1007/978-1-4939-1523-1_20en
dc.identifier.otherORCID: /0000-0003-3873-0650/work/60630840
dc.identifier.urihttps://hdl.handle.net/10023/7944
dc.description.abstractSubcellular, sequence-specific detection of RNA in vivo is a powerful tool to study the macromolecular transport that occurs through plasmodesmata. The RNA-binding domain of Pumilio proteins can be engineered to bind RNA sequences of choice and fused to fluorescent proteins for RNA imaging. This chapter describes the construction of a Pumilio-based imaging system to track the RNA of Tobacco mosaic virus in vivo, and practical aspects of RNA live-cell imaging.
dc.format.extent1178071
dc.language.isoeng
dc.relation.ispartofMethods in Molecular Biologyen
dc.subjectRNA imagingen
dc.subjectPumilo homology domainen
dc.subjectRNA binding proteinen
dc.subjectFluorescence complementationen
dc.subjectProtein engineeringen
dc.subjectFluorescent proteinen
dc.subjectFluorescence microscopyen
dc.subjectTobacco mosaic virusen
dc.subjectQH301 Biologyen
dc.subjectI-PWen
dc.subject.lccQH301en
dc.titlePumilio-based RNA in vivo imagingen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doi10.1007/978-1-4939-1523-1_20
dc.description.statusPeer revieweden
dc.date.embargoedUntil2016-01-01


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