Pumilio-based RNA in vivo imaging
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Subcellular, sequence-specific detection of RNA in vivo is a powerful tool to study the macromolecular transport that occurs through plasmodesmata. The RNA-binding domain of Pumilio proteins can be engineered to bind RNA sequences of choice and fused to fluorescent proteins for RNA imaging. This chapter describes the construction of a Pumilio-based imaging system to track the RNA of Tobacco mosaic virus in vivo, and practical aspects of RNA live-cell imaging.
Tilsner , J 2015 , ' Pumilio-based RNA in vivo imaging ' Methods in Molecular Biology , vol 1217 , pp. 295-328 . DOI: 10.1007/978-1-4939-1523-1_20
Methods in Molecular Biology
© 2015. Springer Science+Business Media New York. The original publication is available at www.springerlink.com
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