Pumilio-based RNA in vivo imaging
Abstract
Subcellular, sequence-specific detection of RNA in vivo is a powerful tool to study the macromolecular transport that occurs through plasmodesmata. The RNA-binding domain of Pumilio proteins can be engineered to bind RNA sequences of choice and fused to fluorescent proteins for RNA imaging. This chapter describes the construction of a Pumilio-based imaging system to track the RNA of Tobacco mosaic virus in vivo, and practical aspects of RNA live-cell imaging.
Citation
Tilsner , J 2015 , ' Pumilio-based RNA in vivo imaging ' , Methods in Molecular Biology , vol. 1217 , pp. 295-328 . https://doi.org/10.1007/978-1-4939-1523-1_20
Publication
Methods in Molecular Biology
Status
Peer reviewed
Type
Journal article
Rights
© 2015. Springer Science+Business Media New York. The original publication is available at www.springerlink.com
Collections
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.