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dc.contributor.authorTilsner, Jens
dc.date.accessioned2015-12-23T00:55:16Z
dc.date.available2015-12-23T00:55:16Z
dc.date.issued2015-04
dc.identifier161706741
dc.identifierf52e2fd0-459d-4246-8975-3c9546948da0
dc.identifier000351381000001
dc.identifier25523910
dc.identifier84924901867
dc.identifier.citationTilsner , J 2015 , ' Techniques for RNA in vivo imaging in plants ' , Journal of Microscopy , vol. 258 , no. 1 , pp. 1-5 . https://doi.org/10.1111/jmi.12208en
dc.identifier.issn0022-2720
dc.identifier.otherORCID: /0000-0003-3873-0650/work/60630853
dc.identifier.urihttps://hdl.handle.net/10023/7929
dc.description.abstractSince the discovery of small RNAs and RNA silencing, RNA biology has taken a centre stage in cell and developmental biology. Small RNAs, but also mRNAs and other types of cellular and viral RNAs are processed at specific subcellular localizations. To fully understand cellular RNA metabolism and the various processes influenced byit, techniques are required that permit the sequence-specific tracking of RNAs in living cells. A variety of methods for RNA visualization have been developed since the 1990s, but plant cells pose particular challenges and not all approaches are applicable to them. On the other hand, plant RNA metabolism is particularly diverse and RNAs are even transported between cells, so RNA imaging can potentially provide many valuable insights into plant function at the cellular and tissue level. This Short Review briefly introduces the currently available techniques for plant RNA in vivo imaging and discusses their suitability for different biological questions.
dc.format.extent5
dc.format.extent604177
dc.language.isoeng
dc.relation.ispartofJournal of Microscopyen
dc.subjectLambda-Nen
dc.subjectPumilioen
dc.subjectMolecular beaconen
dc.subjectMS2en
dc.subjectRNA localisationen
dc.subjectQH301 Biologyen
dc.subject.lccQH301en
dc.titleTechniques for RNA in vivo imaging in plantsen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doi10.1111/jmi.12208
dc.description.statusPeer revieweden
dc.date.embargoedUntil2016-04-01


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